YB-1 binds to CAUC motifs and stimulates exon inclusion by enhancing the recruitment of U2AF to weak polypyrimidine tracts
文献类型：期刊论文
| 作者 | Wei, WJ; Mu, SR; Heiner, M; Fu, X; Cao, LJ; Gong, XF; Bindereif, A; Hui, JY |
| 刊名 | NUCLEIC ACIDS RESEARCH
 |
| 出版日期 | 2012 |
| 卷号 | 40期号:17页码:8622-8636 |
| 通讯作者 | Hui, JY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,jyhui@sibs.ac.cn |
| 英文摘要 | The human Y box-binding protein-1 (YB-1) is a deoxyribonucleic acid (DNA)/ribonucleic acid (RNA)-binding protein with pleiotropic functions. Besides its roles in the regulation of transcription and translation, several recent studies indicate that YB-1 is a spliceosome-associated protein and is involved in alternative splicing, but the underlying mechanism has remained elusive. Here, we define both CAUC and CACC as high-affinity binding motifs for YB-1 by systematic evolution of ligands by exponential enrichment (SELEX) and demonstrate that these newly defined motifs function as splicing enhancers. Interestingly, on the endogenous CD44 gene, YB-1 appears to mediate a network interaction to activate exon v5 inclusion via multiple CAUC motifs in both the alternative exon and its upstream polypyrimidine tract. We provide evidence that YB-1 activates splicing by facilitating the recruitment of U2AF65 to weak polypyrimidine tracts through direct protein-protein interactions. Together, these findings suggest a vital role of YB-1 in activating a subset of weak 3' splice sites in mammalian cells. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | Y-BOX PROTEIN-1 ; ALTERNATIVE SPLICING REGULATION ; COLD SHOCK DOMAIN ; MESSENGER-RNA ; 3'-UNTRANSLATED REGION ; SITE SELECTION ; IDENTIFICATION ; CD44 ; TRANSLATION ; MECHANISMS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000309464300045 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/678]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wei, WJ,Mu, SR,Heiner, M,et al. YB-1 binds to CAUC motifs and stimulates exon inclusion by enhancing the recruitment of U2AF to weak polypyrimidine tracts[J]. NUCLEIC ACIDS RESEARCH,2012,40(17):8622-8636. |
| APA | Wei, WJ.,Mu, SR.,Heiner, M.,Fu, X.,Cao, LJ.,...&Hui, JY.(2012).YB-1 binds to CAUC motifs and stimulates exon inclusion by enhancing the recruitment of U2AF to weak polypyrimidine tracts.NUCLEIC ACIDS RESEARCH,40(17),8622-8636. |
| MLA | Wei, WJ,et al."YB-1 binds to CAUC motifs and stimulates exon inclusion by enhancing the recruitment of U2AF to weak polypyrimidine tracts".NUCLEIC ACIDS RESEARCH 40.17(2012):8622-8636. |
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