Mediator MED23 plays opposing roles in directing smooth muscle cell and adipocyte differentiation
文献类型：期刊论文
| 作者 | Yin, JW; Liang, Y; Park, JY; Chen, DR; Yao, X; Xiao, Q; Liu, Z; Jiang, B; Fu, Y; Bao, MH |
| 刊名 | GENES & DEVELOPMENT
 |
| 出版日期 | 2012 |
| 卷号 | 26期号:19页码:2192-2205 |
| 关键词 | Mediator complex MED23 MAL ELK1 smooth muscle cell adipocyte differentiation |
| 通讯作者 | Wang, G (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, State Key Lab Cell Biol, Shanghai 200031, Peoples R China.,gwang22@sibs.ac.cn |
| 英文摘要 | The Mediator complex functions as a control center, orchestrating diverse signaling, gene activities, and biological processes. However, how Mediator subunits determine distinct cell fates remains to be fully elucidated. Here, we show that Mediator MED23 controls the cell fate preference that directs differentiation into smooth muscle cells (SMCs) or adipocytes. Med23 deficiency facilitates SMC differentiation but represses adipocyte differentiation from the multipotent mesenchymal stem cells. Gene profiling revealed that the presence or absence of Med23 oppositely regulates two sets of genes: the RhoA/MAL targeted cytoskeleton/SMC genes and the Ras/ELK1 targeted growth/adipogenic genes. Mechanistically, MED23 favors ELK1-SRF binding to SMC gene promoters for repression, whereas the lack of MED23 favors MAL-SRF binding to SMC gene promoters for activation. Remarkably, the effect of MED23 on SMC differentiation can be recapitulated in zebrafish embryogenesis. Collectively, our data demonstrate the dual, opposing roles for MED23 in regulating the cytoskeleton/SMC and growth/adipogenic gene programs, suggesting its "Ying-Yang'' function in directing adipogenesis versus SMC differentiation. |
| 学科主题 | Cell Biology; Developmental Biology; Genetics & Heredity |
| 类目[WOS] | Cell Biology ; Developmental Biology ; Genetics & Heredity |
| 关键词[WOS] | SERUM RESPONSE FACTOR ; GENE-EXPRESSION ; MYOCARDIN FAMILY ; TRANSCRIPTIONAL COACTIVATORS ; SIGNAL-TRANSDUCTION ; ADIPOSE-TISSUE ; TARGET GENE ; STEM-CELLS ; COMPLEX ; SRF |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000309514100007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/681]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yin, JW,Liang, Y,Park, JY,et al. Mediator MED23 plays opposing roles in directing smooth muscle cell and adipocyte differentiation[J]. GENES & DEVELOPMENT,2012,26(19):2192-2205. |
| APA | Yin, JW.,Liang, Y.,Park, JY.,Chen, DR.,Yao, X.,...&Wang, G.(2012).Mediator MED23 plays opposing roles in directing smooth muscle cell and adipocyte differentiation.GENES & DEVELOPMENT,26(19),2192-2205. |
| MLA | Yin, JW,et al."Mediator MED23 plays opposing roles in directing smooth muscle cell and adipocyte differentiation".GENES & DEVELOPMENT 26.19(2012):2192-2205. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。