Inhibition of Activity of GABA Transporter GAT1 by delta-Opioid Receptor
文献类型：期刊论文
| 作者 | Pu, L; Xu, NJ; Xia, P; Gu, QB; Ren, SH; Fucke, T; Pei, G; Schwarz, A |
| 刊名 | EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
 |
| 出版日期 | 2012 |
| 期号 | 1页码:818451-818451 |
| 通讯作者 | Schwarz, W (reprint author), Max Planck Inst Biophys, Max von Laue Str 3, D-60438 Frankfurt, Germany.,schwarz@biophys.eu |
| 英文摘要 | Analgesia is a well-documented effect of acupuncture. A critical role in pain sensation plays the nervous system, including the GABAergic system and opioid receptor (OR) activation. Here we investigated regulation of GABA transporter GAT1 by delta OR in rats and in Xenopus oocytes. Synaptosomes of brain from rats chronically exposed to opiates exhibited reduced GABA uptake, indicating that GABA transport might be regulated by opioid receptors. For further investigation we have expressed GAT1 of mouse brain together with mouse delta OR and mu OR in Xenopus oocytes. The function of GAT1 was analyzed in terms of Na+-dependent [H-3] GABA uptake as well as GAT1-mediated currents. Coexpression of delta OR led to reduced number of fully functional GAT1 transporters, reduced substrate translocation, and GAT1-mediated current. Activation of delta OR further reduced the rate of GABA uptake as well as GAT1-mediated current. Coexpression of mu OR, as well as mu OR activation, affected neither the number of transporters, nor rate of GABA uptake, nor GAT1-mediated current. Inhibition of GAT1-mediated current by activation of delta OR was confirmed in whole-cell patch-clamp experiments on rat brain slices of periaqueductal gray. We conclude that inhibition of GAT1 function will strengthen the inhibitory action of the GABAergic system and hence may contribute to acupuncture-induced analgesia. |
| 学科主题 | Integrative & Complementary Medicine |
| 类目[WOS] | Integrative & Complementary Medicine |
| 关键词[WOS] | GAMMA-AMINOBUTYRIC-ACID ; PROTEIN-KINASE-C ; LONG-TERM POTENTIATION ; XENOPUS-OOCYTES ; RAT-BRAIN ; NEUROTRANSMITTER TRANSPORTERS ; ACUPUNCTURE ANALGESIA ; FUNCTIONAL REGULATION ; DOPAMINE TRANSPORTER ; PERIAQUEDUCTAL GRAY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000313187900001 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/703]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Pu, L,Xu, NJ,Xia, P,et al. Inhibition of Activity of GABA Transporter GAT1 by delta-Opioid Receptor[J]. EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE,2012(1):818451-818451. |
| APA | Pu, L.,Xu, NJ.,Xia, P.,Gu, QB.,Ren, SH.,...&Schwarz, A.(2012).Inhibition of Activity of GABA Transporter GAT1 by delta-Opioid Receptor.EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE(1),818451-818451. |
| MLA | Pu, L,et al."Inhibition of Activity of GABA Transporter GAT1 by delta-Opioid Receptor".EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE .1(2012):818451-818451. |
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