Gene transfection and expression in the primary culture of crayfish hemocytes
文献类型：期刊论文
| 作者 | Li, F; Ke, W; Yan, XM; Xu, LM |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2011 |
| 卷号 | 31期号:1页码:161-163 |
| 关键词 | White spot syndrome virus (WSSV) Promoter Primary culture of crayfish hemocytes Electroporation |
| 通讯作者 | Li, F (reprint author), SOA, Inst Oceanog 3, Key Lab Marine Biogenet Resources, 184 Daxue Rd, Xiamen 361005, Peoples R China.,lifang_87@163.com |
| 英文摘要 | Lack of efficient gene delivery and expression methods is the major obstacle for crustacean research in the cellular level. Here, we reported the construction of an expression vector with a strong promoter from shrimp white spot syndrome virus. This vector could efficiently express foreign genes in the primary culture of crayfish hemocytes with the transfection efficiency between 5 and 10% by electroporation. Our findings provide a method for in vitro gene functional study in primary crayfish cells. (C) 2011 Elsevier Ltd. All rights reserved. |
| 学科主题 | Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences |
| 类目[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 关键词[WOS] | SPOT SYNDROME VIRUS ; IMMEDIATE-EARLY GENES ; PENAEUS-MONODON ; SHRIMP |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000292407900020 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/718]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, F,Ke, W,Yan, XM,et al. Gene transfection and expression in the primary culture of crayfish hemocytes[J]. FISH & SHELLFISH IMMUNOLOGY,2011,31(1):161-163. |
| APA | Li, F,Ke, W,Yan, XM,&Xu, LM.(2011).Gene transfection and expression in the primary culture of crayfish hemocytes.FISH & SHELLFISH IMMUNOLOGY,31(1),161-163. |
| MLA | Li, F,et al."Gene transfection and expression in the primary culture of crayfish hemocytes".FISH & SHELLFISH IMMUNOLOGY 31.1(2011):161-163. |
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