H-1, N-15 chemical shift assignments of the imino groups in the base pairs of Escherichia coli tRNA(Leu) (CAG)
文献类型：期刊论文
| 作者 | Hao, ZX; Feng, R; Wang, ED; Zhu, GA |
| 刊名 | BIOMOLECULAR NMR ASSIGNMENTS
 |
| 出版日期 | 2011 |
| 卷号 | 5期号:1页码:71-74 |
| 关键词 | tRNA Imino groups Chemical shift Assignment NMR |
| 通讯作者 | Wang, ED (reprint author), Chinese Acad Sci, Grad Sch, Shanghai Inst Biol Sci, State Key Lab Mol Biol,Inst Biochem & Cell Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,gzhu@ust.hk |
| 英文摘要 | tRNA molecules are the adaptors in ribosome-based protein biosynthesis and are stabilized by Mg2+. However, the detailed mechanism for the Mg2+ mediated stability is not fully understood. To study the effects of Mg2+ on conformational flexibility of Escherichia coli tRNA(Leu) (CAG) at millisecond timescale, we applied NMR spectroscopic approach to measure proton exchange rates of imino groups in the presence of different concentration of Mg2+ and correlated them with the corresponding aminoacylation activity of tRNA(Leu). Here, we report the first part of the above mentioned study, the H-1, N-15 chemical shift assignments of the imino groups in all base pairs of Escherichia coli tRNA(Leu) (CAG) based on 2D H-1-N-15 TROSY, 2D NOESY and 3D NOESY-HMQC experiments. This work laid the foundation for the NMR study of tRNA(Leu) (BMRB deposits with accession number 17078). |
| 学科主题 | Biophysics; Spectroscopy |
| 类目[WOS] | Biophysics ; Spectroscopy |
| 关键词[WOS] | NUCLEAR MAGNETIC-RESONANCE ; TRANSFER RNAPHE ; PURIFICATION ; PROTEIN ; SIGNALS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000288076300018 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/720]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Hao, ZX,Feng, R,Wang, ED,et al. H-1, N-15 chemical shift assignments of the imino groups in the base pairs of Escherichia coli tRNA(Leu) (CAG)[J]. BIOMOLECULAR NMR ASSIGNMENTS,2011,5(1):71-74. |
| APA | Hao, ZX,Feng, R,Wang, ED,&Zhu, GA.(2011).H-1, N-15 chemical shift assignments of the imino groups in the base pairs of Escherichia coli tRNA(Leu) (CAG).BIOMOLECULAR NMR ASSIGNMENTS,5(1),71-74. |
| MLA | Hao, ZX,et al."H-1, N-15 chemical shift assignments of the imino groups in the base pairs of Escherichia coli tRNA(Leu) (CAG)".BIOMOLECULAR NMR ASSIGNMENTS 5.1(2011):71-74. |
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