AMID Mediates Adenosine-Induced Caspase-Independent HuH-7 Cell Apoptosis
文献类型：期刊论文
| 作者 | Yang, DQ; Yaguchi, T; Nagata, T; Gotoh, A; Dovat, S; Song, CH; Nishizaki, T |
| 刊名 | CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
 |
| 出版日期 | 2011 |
| 卷号 | 27期号:1页码:37-44 |
| 关键词 | Adenosine AMID Caspase-independent Apoptosis HuH-7 cell |
| 通讯作者 | Nishizaki, T (reprint author), Hyogo Coll Med, Dept Physiol, Div Bioinformat, 1-1 Mukogawa Cho, Nishinomiya, Hyogo 6638501, Japan.,tomoyuki@hyo-med.ac.jp |
| 英文摘要 | Background/Aims: The mechanism underlying extra-cellular adenosine-induced caspase-independent apoptosis in HuH-7 human hepatoma cells is not fully understood. The present study investigated the role for apoptosis-inducing factor (AIF)-homologous mitochondrion-associated inducer of death (AMID) in the pathway. Methods: To see the implication of AMID in adenosine-induced HuH-7 cell apoptosis, real-time reverse transcription-polymerase chain reaction (RT-PCR), immunofluorescent cytochemistry, time-laps GFP monitoring, cell cycle analysis, flow cytometry, Western blotting, cell viability assay, and TUNEL staining were carried out. Results: Adenosine upregulated AMID expression in HuH-7 cells, and translocated AMID from the cytosol into the nucleus. Adenosine induced HuH-7 cell apoptosis, and the effect was further enhanced by overexpressing AMID. Adenosine-induced HuH-7 cell apoptosis, alternatively, was inhibited by knocking-down AMID. Conclusion: The results of the present study provide evidence for AMID as a critical factor for adenosine-induced caspase-independent HuH-7 cell apoptosis. Copyright (C) 2011 S. Karger AG, Basel |
| 学科主题 | Cell Biology; Physiology |
| 类目[WOS] | Cell Biology ; Physiology |
| 关键词[WOS] | HUMAN HEPATOMA-CELLS ; CANCER CELLS ; RECEPTOR AGONIST ; PROTEIN-KINASE ; DNA-BINDING ; FACTOR AIF ; IB-MECA ; ACTIVATION ; DEATH ; EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000288585000006 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/727]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yang, DQ,Yaguchi, T,Nagata, T,et al. AMID Mediates Adenosine-Induced Caspase-Independent HuH-7 Cell Apoptosis[J]. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY,2011,27(1):37-44. |
| APA | Yang, DQ.,Yaguchi, T.,Nagata, T.,Gotoh, A.,Dovat, S.,...&Nishizaki, T.(2011).AMID Mediates Adenosine-Induced Caspase-Independent HuH-7 Cell Apoptosis.CELLULAR PHYSIOLOGY AND BIOCHEMISTRY,27(1),37-44. |
| MLA | Yang, DQ,et al."AMID Mediates Adenosine-Induced Caspase-Independent HuH-7 Cell Apoptosis".CELLULAR PHYSIOLOGY AND BIOCHEMISTRY 27.1(2011):37-44. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。