Adenosine Activates AMPK to Phosphorylate Bcl-X-L Responsible for Mitochondrial Damage and DIABLO Release in HuH-7 Cells
文献类型：期刊论文
| 作者 | Yang, DQ; Yaguchi, T; Nakano, T; Nishizaki, T |
| 刊名 | CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
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| 出版日期 | 2011 |
| 卷号 | 27期号:1页码:71-78 |
| 关键词 | Adenosine AMPK Bcl-X-L Phosphorylation Mitochondria DIABLO Apoptosis HuH-7 cell |
| 通讯作者 | Nishizaki, T (reprint author), Hyogo Coll Med, Dept Physiol, Div Bioinformat, 1-1 Mukogawa Cho, Nishinomiya, Hyogo 6638501, Japan.,tomoyuki@hyo-med.ac.jp |
| 英文摘要 | Background/Aims: Accumulating evidence has pointed to AMP-activated protein kinase (AMPK) as an inducer of apoptosis in a variety of cancer cells. The present study aimed at understanding AMPK signals for adenosine-induced HuH-7 cell apoptosis. Methods: Cell viability, AMPK activity, mitochondrial membrane potentials, phosphorylation of Bcl-X-L, in situ DIABLO mobilizations, and caspase-3 activity were monitored in HuH-7 cells. Plasmid DNAs for DIABLO-GFP, mutant Bcl-X-L, dominant negative mutant AMPK alpha 2 and the siRNAs to silence the AMPK alpha 1 or AMPK alpha 2 targeted gene were constructed and transfected. Results: Adenosine or the AMPK activator AICAR induced apoptosis in HuH-7 cells, and no synergistic effect was obtained with co-treatment. Adenosine activated AMPK, to phosphorylate Bcl-X-L. Adenosine or AICAR disrupted mitochondrial membrane potentials, and the effect was inhibited by knocking-down AMPK alpha 1 and/or AMPK alpha 2, expressing dominant negative mutant AMPK alpha 2 or mutant Bcl-X-L lacking Ser/Thr phosphorylation sites. AICAR stimulated DIABLO release from the mitochondria, and the release was suppressed by expressing the mutant Bcl-X-L. AICAR activated caspase-3, which was also inhibited by expressing the mutant Bcl-X-L. Conclusion: Adenosine activates AMPK, to disrupt mitochondrial membrane potentials through Bcl-X-L phosphorylation, allowing DIABLO release from the mitochondria, as a factor for caspase-3 activation to induce HuH-7 cell apoptosis. Copyright (C) 2011 S. Karger AG, Basel |
| 学科主题 | Cell Biology; Physiology |
| 类目[WOS] | Cell Biology ; Physiology |
| 关键词[WOS] | HUMAN HEPATOMA-CELLS ; PROTEIN-KINASE ; APOPTOSIS ; DEATH ; EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000288585000010 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/728]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yang, DQ,Yaguchi, T,Nakano, T,et al. Adenosine Activates AMPK to Phosphorylate Bcl-X-L Responsible for Mitochondrial Damage and DIABLO Release in HuH-7 Cells[J]. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY,2011,27(1):71-78. |
| APA | Yang, DQ,Yaguchi, T,Nakano, T,&Nishizaki, T.(2011).Adenosine Activates AMPK to Phosphorylate Bcl-X-L Responsible for Mitochondrial Damage and DIABLO Release in HuH-7 Cells.CELLULAR PHYSIOLOGY AND BIOCHEMISTRY,27(1),71-78. |
| MLA | Yang, DQ,et al."Adenosine Activates AMPK to Phosphorylate Bcl-X-L Responsible for Mitochondrial Damage and DIABLO Release in HuH-7 Cells".CELLULAR PHYSIOLOGY AND BIOCHEMISTRY 27.1(2011):71-78. |
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