Modular pathways for editing non-cognate amino acids by human cytoplasmic leucyl-tRNA synthetase
文献类型：期刊论文
| 作者 | Chen, X; Ma, JJ; Tan, M; Yao, P; Hu, QH; Eriani, G; Wang, ED |
| 刊名 | NUCLEIC ACIDS RESEARCH
 |
| 出版日期 | 2011 |
| 卷号 | 39期号:1页码:235-247 |
| 通讯作者 | Wang, ED (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol,Grad Sch, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,edwang@sibs.ac.cn |
| 英文摘要 | To prevent potential errors in protein synthesis, some aminoacyl-transfer RNA (tRNA) synthetases have evolved editing mechanisms to hydrolyze misactivated amino acids (pre-transfer editing) or misacylated tRNAs (post-transfer editing). Class Ia leucyl-tRNA synthetase (LeuRS) may misactivate various natural and non-protein amino acids and then mischarge tRNA(Leu). It is known that the fidelity of prokaryotic LeuRS depends on multiple editing pathways to clear the incorrect intermediates and products in the every step of aminoacylation reaction. Here, we obtained human cytoplasmic LeuRS (hcLeuRS) and tRNA(Leu) (hctRNA(Leu)) with high activity from Escherichia coli overproducing strains to study the synthetic and editing properties of the enzyme. We revealed that hcLeuRS could adjust its editing strategy against different non-cognate amino acids. HcLeuRS edits norvaline predominantly by post-transfer editing; however, it uses mainly pre-transfer editing to edit a-amino butyrate, although both amino acids can be charged to tRNA(Leu). Post-transfer editing as a final checkpoint of the reaction was very important to prevent mis-incorporation in vitro. These results provide insight into the modular editing pathways created to prevent genetic code ambiguity by evolution. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | I TRANSFER-RNA ; GENETIC-CODE AMBIGUITY ; ESCHERICHIA-COLI ; CELL VIABILITY ; PROTEIN-SYNTHESIS ; STRUCTURAL BASIS ; COMPLEX ; AMINOACYLATION ; DISCRIMINATION ; SPECIFICITY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000286008500024 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/733]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Chen, X,Ma, JJ,Tan, M,et al. Modular pathways for editing non-cognate amino acids by human cytoplasmic leucyl-tRNA synthetase[J]. NUCLEIC ACIDS RESEARCH,2011,39(1):235-247. |
| APA | Chen, X.,Ma, JJ.,Tan, M.,Yao, P.,Hu, QH.,...&Wang, ED.(2011).Modular pathways for editing non-cognate amino acids by human cytoplasmic leucyl-tRNA synthetase.NUCLEIC ACIDS RESEARCH,39(1),235-247. |
| MLA | Chen, X,et al."Modular pathways for editing non-cognate amino acids by human cytoplasmic leucyl-tRNA synthetase".NUCLEIC ACIDS RESEARCH 39.1(2011):235-247. |
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