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Genomewide characterization of non-polyadenylated RNAs
文献类型：期刊论文
| 作者 | Yang, L; Duff, MO; Graveley, BR; Carmichael, GG; Chen, LL |
| 刊名 | GENOME BIOLOGY
 |
| 出版日期 | 2011 |
| 卷号 | 12期号:2页码:R16-R16 |
| 通讯作者 | Chen, LL (reprint author), Univ Connecticut, Ctr Hlth, Dept Genet & Dev Biol, Stem Cell Inst, 263 Farmington Ave, Farmington, CT 06030 USA.,linglingchen@sibcb.ac.cn |
| 英文摘要 | Background: RNAs can be physically classified into poly(A)+ or poly(A)- transcripts according to the presence or absence of a poly(A) tail at their 3' ends. Current deep sequencing approaches largely depend on the enrichment of transcripts with a poly(A) tail, and therefore offer little insight into the nature and expression of transcripts that lack poly(A) tails. Results: We have used deep sequencing to explore the repertoire of both poly(A)+ and poly(A)- RNAs from HeLa cells and H9 human embryonic stem cells (hESCs). Using stringent criteria, we found that while the majority of transcripts are poly(A)+, a significant portion of transcripts are either poly(A)- or bimorphic, being found in both the poly(A)+ and poly(A) populations. Further analyses revealed that many mRNAs may not contain classical long poly(A) tails and such messages are overrepresented in specific functional categories. In addition, we surprisingly found that a few excised introns accumulate in cells and thus constitute a new class of non-polyadenylated long non-coding RNAs. Finally, we have identified a specific subset of poly(A)- histone mRNAs, including two histone H1 variants, that are expressed in undifferentiated hESCs and are rapidly diminished upon differentiation; further, these same histone genes are induced upon reprogramming of fibroblasts to induced pluripotent stem cells. Conclusions: We offer a rich source of data that allows a deeper exploration of the poly(A)- landscape of the eukaryotic transcriptome. The approach we present here also applies to the analysis of the poly(A)- transcriptomes of other organisms. |
| 学科主题 | Biotechnology & Applied Microbiology; Genetics & Heredity |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| 关键词[WOS] | EMBRYONIC STEM-CELLS ; MESSENGER-RNAS ; NONCODING RNA ; POLY(A) TAIL ; CHROMATIN ; CYCLE ; TRANSCRIPTOME ; METABOLISM ; RESOLUTION ; HISTONES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000289365600012 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/734]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yang, L,Duff, MO,Graveley, BR,et al. Genomewide characterization of non-polyadenylated RNAs[J]. GENOME BIOLOGY,2011,12(2):R16-R16. |
| APA | Yang, L,Duff, MO,Graveley, BR,Carmichael, GG,&Chen, LL.(2011).Genomewide characterization of non-polyadenylated RNAs.GENOME BIOLOGY,12(2),R16-R16. |
| MLA | Yang, L,et al."Genomewide characterization of non-polyadenylated RNAs".GENOME BIOLOGY 12.2(2011):R16-R16. |
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