The human LIS1 is downregulated in hepatocellular carcinoma and plays a tumor suppressor function
文献类型：期刊论文
| 作者 | Xing, Z; Tang, X; Gao, Y; Da, L; Song, H; Wang, SQ; Tiollais, P; Li, TP; Zhao, MJ |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2011 |
| 卷号 | 409期号:2页码:193-199 |
| 关键词 | LIS1 gene Reduced expression HCC Cell transformation Mitotic defects |
| 通讯作者 | Zhao, MJ (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,mjzhao@sibs.ac.cn |
| 英文摘要 | The human lissencephaly-1 gene (LIS1) is a disease gene responsible for Miller-Dieker lissencephaly syndrome (MDL). LIS1 gene is located in the region of chromosome 17p13.3 that is frequency deleted in MDL patients and in human liver cancer cells. However, the expression and significance of LIS1 in liver cancer remain unknown. Here, we investigated the expression of LIS1 in hepatocellular carcinoma (HCC) tissues by real-time PCR, Western blot, and immunohistochemistry. The results indicated that the mRNA and protein levels of LIS1 were downregulated in about 70% of HCC tissues, and this downregulation was significantly associated with tumor progression. Functional studies showed that the reduction of LIS1 expression in the normal human liver cell line QSG7701 or the mouse fibroblast cell line NIH3T3 by shRNA resulted in colony formation in soft agar and xenograft tumor formation in nude mice, demonstrating that a decrease in the LIS1 level can promote the oncogenic transformation of cells. We also observed that the phenotypes of LIS1-knockdown cells displayed various defective mitotic structures, suggesting that the mechanism by which reduced LIS1 levels results in tumorigenesis is associated with its role in mitosis. Furthermore, we demonstrated that ectopic expression of LIS1 could significantly inhibit HCC cell proliferation and colony formation. Our results suggest that LIS1 plays a potential tumor suppressor role in the development and progression of HCC. (C) 2011 Elsevier Inc. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | CHROMOSOME 17P13.3 ; LISSENCEPHALY GENE ; LIS1/DYNACTIN ; INSTABILITY ; NEUROBLASTS ; CHECKPOINT ; DYNEIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000291779100008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/738]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Xing, Z,Tang, X,Gao, Y,et al. The human LIS1 is downregulated in hepatocellular carcinoma and plays a tumor suppressor function[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2011,409(2):193-199. |
| APA | Xing, Z.,Tang, X.,Gao, Y.,Da, L.,Song, H.,...&Zhao, MJ.(2011).The human LIS1 is downregulated in hepatocellular carcinoma and plays a tumor suppressor function.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,409(2),193-199. |
| MLA | Xing, Z,et al."The human LIS1 is downregulated in hepatocellular carcinoma and plays a tumor suppressor function".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 409.2(2011):193-199. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。