MicroRNAs modulate the Wnt signaling pathway through targeting its inhibitors
文献类型：期刊论文
| 作者 | Liu, YG; Huang, TW; Zhao, XL; Cheng, LP |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2011 |
| 卷号 | 408期号:2页码:259-264 |
| 关键词 | MicroRNA Inhibitors Wnt/beta-catenin pathway Gsk3b Wnt1-cre Dicer (Dicer1) conditional knockout |
| 通讯作者 | Cheng, LP (reprint author), Chinese Acad Sci, State Key Lab Neurosci, Inst Neurosci, Shanghai Inst Biol Sci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,lpcheng@ion.ac.cn |
| 英文摘要 | Increasing evidence indicates that microRNAs (miRNAs) play important roles in mouse brain development. We and several other reports recently have demonstrated that Wnt1-cre-mediated loss of Dicer, the key enzyme for miRNA biosynthesis, results in malformation of the midbrain and cerebellum and failure of neural crest and dopaminergic differentiation. The underlying mechanisms, however, remain poorly understood. The resemblance of some of the phenotypes in the Wnt1-cre Dicer conditional knockout embryos and Wnt1(-/-),Wnt1(-/-);Wnt3(-/-) and Wnt1-cre;beta-cateninn(flox/flox) knockout embryos reminds us that loss of miRNA may disrupt the Wnt-beta-catenin signaling. Here we provide evidence that miRNAs modulate the Wnt signaling pathway through targeting its inhibitors. First, we predicted miRNA binding sites in the 3' UTRs of candidate inhibitors of the Wnt signaling pathway and luciferase assays revealed that several inhibitors of Wnt signaling pathway were targeted by miRNAs. Second, we demonstrated that several miRNAs could modulate the expression of Gsk3b. an inhibitor of Wnt signaling, post-transcriptional in 293T cells. Third, we found that several miRNAs were able to regulate the Wnt-beta-catenin signaling activity in 293T cells. More interestingly, the expression of beta-catenin protein was dramatically reduced in the Wnt1-cre-meidiated Dicer knockout brain tissue compared with control. Our studies therefore suggest that miRNAs might exert their functions, at least in part, by modulating the Wnt signaling pathway through targeting its inhibitors. (C) 2011 Elsevier Inc. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | NEURAL CREST ; BETA-CATENIN ; INT-1 PROTOONCOGENE ; MIDBRAIN ; MALFORMATION ; PROGENITORS ; FAILURE ; BRAIN ; DICER ; GENE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000290606200012 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/740]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Liu, YG,Huang, TW,Zhao, XL,et al. MicroRNAs modulate the Wnt signaling pathway through targeting its inhibitors[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2011,408(2):259-264. |
| APA | Liu, YG,Huang, TW,Zhao, XL,&Cheng, LP.(2011).MicroRNAs modulate the Wnt signaling pathway through targeting its inhibitors.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,408(2),259-264. |
| MLA | Liu, YG,et al."MicroRNAs modulate the Wnt signaling pathway through targeting its inhibitors".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 408.2(2011):259-264. |
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