Expression, purification, crystallization, and preliminary X-ray diffraction analysis of the human TLE1 Q domain
文献类型：期刊论文
| 作者 | Wang, S; Du, JM; Tang, H; Ding, XY; Zha, MW; Xu, ZF |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2011 |
| 卷号 | 43期号:2页码:149-153 |
| 关键词 | crystallization glutamine LEF1 Notch selenomethionine TLE1 X-ray prokaryotic expression |
| 通讯作者 | Zha, MW (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Res Ctr Struct Biol, Shanghai 200031, Peoples R China.,mwzha@sibs.ac.cn ; xu_zhi_fei@yahoo.com.cn |
| 英文摘要 | Human transducin-like enhancer of split 1 (TLE1) plays crucial roles in a number of developmental processes and is involved in pathogenesis of malignancy tumors. The N-terminal glutamine-rich domain (Q domain) of TLE1 mediates its tetramerization and interactions with different DNA-binding transcription factors to regulate Notch and Wnt signaling pathways. To better understand the molecular mechanism of TLE1's functions in these pathways, we cloned, purified, and crystallized the TLE1 Q domain (TLE1-Q). The crystals belong to space group C222(1), with the complete diffraction data of the native and Se-Met TLE1-Q collected to 3.5 and 4.1 angstrom resolutions, respectively. The phasing-solving and model building are in progress. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | TRANSCRIPTIONAL REPRESSION ; BETA-CATENIN ; GROUCHO ; PROTEINS ; COREPRESSORS ; INTERACT ; FAMILY ; MODEL ; ACTS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000286670900009 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/747]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, S,Du, JM,Tang, H,et al. Expression, purification, crystallization, and preliminary X-ray diffraction analysis of the human TLE1 Q domain[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2011,43(2):149-153. |
| APA | Wang, S,Du, JM,Tang, H,Ding, XY,Zha, MW,&Xu, ZF.(2011).Expression, purification, crystallization, and preliminary X-ray diffraction analysis of the human TLE1 Q domain.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,43(2),149-153. |
| MLA | Wang, S,et al."Expression, purification, crystallization, and preliminary X-ray diffraction analysis of the human TLE1 Q domain".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 43.2(2011):149-153. |
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