A conserved motif within RAP1 has diversified roles in telomere protection and regulation in different organisms
文献类型：期刊论文
| 作者 | Chen, Y; Rai, R; Zhou, ZR; Kanoh, J; Ribeyre, C; Yang, YT; Zheng, H; Damay, P; Wang, F; Tsujii, H |
| 刊名 | NATURE STRUCTURAL & MOLECULAR BIOLOGY
 |
| 出版日期 | 2011 |
| 卷号 | 18期号:2页码:213-U282 |
| 通讯作者 | Lei, M (reprint author), Univ Michigan, Sch Med, Howard Hughes Med Inst, Ann Arbor, MI 48109 USA.,s.chang@yale.edu ; leim@umich.edu |
| 英文摘要 | Repressor activator protein 1 (RAP1) is the most highly conserved telomere protein. It is involved in protecting chromosome ends in fission yeast and promoting gene silencing in Saccharomyces cerevisiae, whereas it represses homology-directed recombination at telomeres in mammals. To understand how RAP1 has such diverse functions at telomeres, we solved the crystal or solution structures of the RAP1 C-terminal (RCT) domains of RAP1 from multiple organisms in complex with their respective protein-binding partners. Our analysis establishes RAP1(RCT) as an evolutionarily conserved protein-protein interaction module. In mammalian and fission yeast cells, this module interacts with TRF2 and Taz1, respectively, targeting RAP1 to chromosome ends for telomere protection. In contrast, S. cerevisiae RAP1 uses its RCT domain to recruit Sir3 to telomeres to mediate gene silencing. Together, our results show that, depending on the organism, the evolutionarily conserved RAP1 RCT motif has diverse functional roles at telomeres. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
| 关键词[WOS] | NMR STRUCTURE CALCULATION ; SINGLE-STRANDED-DNA ; SACCHAROMYCES-CEREVISIAE ; FISSION YEAST ; SCHIZOSACCHAROMYCES-POMBE ; HOMOLOGOUS RECOMBINATION ; DYSFUNCTIONAL TELOMERES ; CRYSTAL-STRUCTURE ; DAMAGE RESPONSE ; NOE ASSIGNMENT |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000286969200015 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/752]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Chen, Y,Rai, R,Zhou, ZR,et al. A conserved motif within RAP1 has diversified roles in telomere protection and regulation in different organisms[J]. NATURE STRUCTURAL & MOLECULAR BIOLOGY,2011,18(2):213-U282. |
| APA | Chen, Y.,Rai, R.,Zhou, ZR.,Kanoh, J.,Ribeyre, C.,...&Lei, M.(2011).A conserved motif within RAP1 has diversified roles in telomere protection and regulation in different organisms.NATURE STRUCTURAL & MOLECULAR BIOLOGY,18(2),213-U282. |
| MLA | Chen, Y,et al."A conserved motif within RAP1 has diversified roles in telomere protection and regulation in different organisms".NATURE STRUCTURAL & MOLECULAR BIOLOGY 18.2(2011):213-U282. |
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