A Simple Method to Raise Efficiency of Interaction With Nucleic Acids of Self Polymerization-prone Proteins
文献类型：期刊论文
| 作者 | Wang, Y; Sun, DQ; Liu, DG |
| 刊名 | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
 |
| 出版日期 | 2011 |
| 卷号 | 38期号:2页码:173-176 |
| 关键词 | self polymerization inhibition interactions between nucleic acids and proteins fluorescein isothiocyanate |
| 通讯作者 | Liu, DG (reprint author), Chinese Acad Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,dgliu@sibs.ac.cn |
| 英文摘要 | A basic research subject is the study on interactions between proteins, DNA and RNA. Majority of DNA and RNA-binding proteins are self polymerization-prone and may form aggregates that are difficult to bind DNA or RNA, thus hampering experiments. Labeling the proteins with fluorescein isothiocyanate significantly inhibits this tendency and markedly increases the binding efficiency of these proteins to bind nucleic acid molecules. This simple method has been utilized in the investigation on binding of C/EBP beta 3' UTR RNA by cytokeratin 18. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000287791700010 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/754]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, Y,Sun, DQ,Liu, DG. A Simple Method to Raise Efficiency of Interaction With Nucleic Acids of Self Polymerization-prone Proteins[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2011,38(2):173-176. |
| APA | Wang, Y,Sun, DQ,&Liu, DG.(2011).A Simple Method to Raise Efficiency of Interaction With Nucleic Acids of Self Polymerization-prone Proteins.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,38(2),173-176. |
| MLA | Wang, Y,et al."A Simple Method to Raise Efficiency of Interaction With Nucleic Acids of Self Polymerization-prone Proteins".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 38.2(2011):173-176. |
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