Slit-2 repels the migration of olfactory ensheathing cells by triggering Ca2+-dependent cofilin activation and RhoA inhibition
文献类型：期刊论文
| 作者 | Huang, ZH; Wang, Y; Su, ZD; Geng, JG; Chen, YZ; Yuan, XB; He, C |
| 刊名 | JOURNAL OF CELL SCIENCE
 |
| 出版日期 | 2011 |
| 卷号 | 124期号:2页码:186-197 |
| 关键词 | Ca2+ Cofilin Migration Olfactory ensheathing cells RhoA Slit-2 |
| 通讯作者 | Yuan, XB (reprint author), Chinese Acad Sci, Inst Neurosci, Shanghai 200031, Peoples R China.,yuanxb@ion.ac.cn ; chenghe@smmu.edu.cn |
| 英文摘要 | Olfactory ensheathing cells (OECs) migrate from the olfactory epithelium towards the olfactory bulb during development. However, the guidance mechanism for OEC migration remains a mystery. Here we show that migrating OECs expressed the receptor of the repulsive guidance factor Slit-2. A gradient of Slit-2 in front of cultured OECs first caused the collapse of the leading front, then the reversal of cell migration. These Slit-2 effects depended on the Ca2+ release from internal stores through inositol (1,4,5)-triphosphate receptor channels. Interestingly, in response to Slit-2 stimulation, collapse of the leading front required the activation of the F-actin severing protein cofilin in a Ca2+-dependent manner, whereas the subsequent reversal of the soma migration depended on the reversal of RhoA activity across the cell. Finally, the Slit-2-induced repulsion of cell migration was fully mimicked by co-application of inhibitors of F-actin polymerization and RhoA kinase. Our findings revealed Slit-2 as a repulsive guidance factor for OEC migration and an unexpected link between Ca2+ and cofilin signaling during Slit-2-triggered repulsion. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | ACTIN-FILAMENT TURNOVER ; LIM-KINASE ; GROWTH CONE ; NEURONAL MIGRATION ; SLINGSHOT PHOSPHATASE ; AXON GUIDANCE ; NEURITE OUTGROWTH ; FAMILY GTPASES ; SPINAL-CORD ; IN-VITRO |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000285634000007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/757]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Huang, ZH,Wang, Y,Su, ZD,et al. Slit-2 repels the migration of olfactory ensheathing cells by triggering Ca2+-dependent cofilin activation and RhoA inhibition[J]. JOURNAL OF CELL SCIENCE,2011,124(2):186-197. |
| APA | Huang, ZH.,Wang, Y.,Su, ZD.,Geng, JG.,Chen, YZ.,...&He, C.(2011).Slit-2 repels the migration of olfactory ensheathing cells by triggering Ca2+-dependent cofilin activation and RhoA inhibition.JOURNAL OF CELL SCIENCE,124(2),186-197. |
| MLA | Huang, ZH,et al."Slit-2 repels the migration of olfactory ensheathing cells by triggering Ca2+-dependent cofilin activation and RhoA inhibition".JOURNAL OF CELL SCIENCE 124.2(2011):186-197. |
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