Flotillins play an essential role in Niemann-Pick C1-like 1-mediated cholesterol uptake
文献类型：期刊论文
| 作者 | Ge, LA; Qi, W; Wang, LJ; Miao, HH; Qu, YX; Li, BL; Song, BL |
| 刊名 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
 |
| 出版日期 | 2011 |
| 卷号 | 108期号:2页码:551-556 |
| 通讯作者 | Song, BL (reprint author), Chinese Acad Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,blsong@sibs.ac.cn |
| 英文摘要 | Dietary absorption is a major way for mammals to obtain cholesterol, which is mediated by Niemann-Pick C1-like 1 (NPC1L1) via vesicular endocytosis. One fundamental question in this process is how free cholesterol is efficiently taken up through the internalization of NPC1L1. Using exogenously expressed NPC1L1-EGFP, we show that the lipid raft proteins flotillins associate with NPC1L1 and their localization is regulated by NPC1L1 during intracellular trafficking. Furthermore, flotillins are essential for NPC1L1-mediated cellular cholesterol uptake, biliary cholesterol reabsorption, and the regulation of lipid levels in mice. Together with NPC1L1, they form cholesterol-enriched membrane microdomains, which function as carriers for bulk of cholesterol. The hypocholesterolemic drug ezetimibe disrupts the association between NPC1L1 and flotillins, which blocks the formation of the cholesterol-enriched microdomains. Our findings reveal a functional role of flotillins in NPC1L1-mediated cholesterol uptake and elucidate the formation of NPC1L1-flotillins-postive cholesterol-enriched membrane microdomains as a mechanism for efficient cholesterol absorption. |
| 学科主题 | Science & Technology - Other Topics |
| 类目[WOS] | Multidisciplinary Sciences |
| 关键词[WOS] | REGGIE/FLOTILLIN PROTEINS ; REGULATED TRANSLOCATION ; CELL-SURFACE ; NPC1L1 ; MEMBRANE ; MICRODOMAINS ; ABSORPTION ; EZETIMIBE ; CAVEOLAE ; RAFTS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000286097700026 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/758]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Ge, LA,Qi, W,Wang, LJ,et al. Flotillins play an essential role in Niemann-Pick C1-like 1-mediated cholesterol uptake[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2011,108(2):551-556. |
| APA | Ge, LA.,Qi, W.,Wang, LJ.,Miao, HH.,Qu, YX.,...&Song, BL.(2011).Flotillins play an essential role in Niemann-Pick C1-like 1-mediated cholesterol uptake.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,108(2),551-556. |
| MLA | Ge, LA,et al."Flotillins play an essential role in Niemann-Pick C1-like 1-mediated cholesterol uptake".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 108.2(2011):551-556. |
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