Modification of Nonstructural Protein 1 of Influenza A Virus by SUMO1
文献类型：期刊论文
| 作者 | Xu, K; Klenk, C; Liu, B; Keiner, B; Cheng, JK; Zheng, BJ; Li, L; Han, QL; Wang, C; Li, TX |
| 刊名 | JOURNAL OF VIROLOGY
 |
| 出版日期 | 2011 |
| 卷号 | 85期号:2页码:1086-1098 |
| 通讯作者 | Klenk, HD (reprint author), Univ Marburg, Inst Virol, Hans Meerwein Str 2, D-35043 Marburg, Germany.,klenk@staff.uni-marburg.de ; bsun@sibs.ac.cn |
| 英文摘要 | Nonstructural protein 1 (NS1) is one of the major factors resulting in the efficient infection rate and high level of virulence of influenza A virus. Although consisting of only approximately 230 amino acids, NS1 has the ability to interfere with several systems of the host viral defense. In the present study, we demonstrate that NS1 of the highly pathogenic avian influenza A/Duck/Hubei/L-1/2004 (H5N1) virus interacts with human Ubc9, which is the E2 conjugating enzyme for sumoylation, and we show that SUMO1 is conjugated to H5N1 NS1 in both transfected and infected cells. Furthermore, two lysine residues in the C terminus of NS1 were identified as SUMO1 acceptor sites. When the SUMO1 acceptor sites were removed by mutation, NS1 underwent rapid degradation. Studies of different influenza A virus strains of human and avian origin showed that the majority of viruses possess an NS1 protein that is modified by SUMO1, except for the recently emerged swine-origin influenza A virus (S-OIV) (H1N1). Interestingly, growth of a sumoylation-deficient WSN virus mutant was retarded compared to that of wild-type virus. Together, these results indicate that sumoylation enhances NS1 stability and thus promotes rapid growth of influenza A virus. |
| 学科主题 | Virology |
| 类目[WOS] | Virology |
| 关键词[WOS] | PRE-MESSENGER-RNAS ; NS1 PROTEIN ; TRANSCRIPTIONAL REGULATION ; ANTIVIRAL RESPONSES ; INFECTED-CELLS ; H1N1 INFECTION ; SUMOYLATION ; INHIBITION ; INTERFERON ; BINDING |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000285554300043 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/759]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Xu, K,Klenk, C,Liu, B,et al. Modification of Nonstructural Protein 1 of Influenza A Virus by SUMO1[J]. JOURNAL OF VIROLOGY,2011,85(2):1086-1098. |
| APA | Xu, K.,Klenk, C.,Liu, B.,Keiner, B.,Cheng, JK.,...&Sun, B.(2011).Modification of Nonstructural Protein 1 of Influenza A Virus by SUMO1.JOURNAL OF VIROLOGY,85(2),1086-1098. |
| MLA | Xu, K,et al."Modification of Nonstructural Protein 1 of Influenza A Virus by SUMO1".JOURNAL OF VIROLOGY 85.2(2011):1086-1098. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。