Transcription profiling of eight cytochrome P450s potentially involved in xenobiotic metabolism in the silkworm, Bombyx mori
文献类型：期刊论文
| 作者 | Zhao, GD; Zhao, SS; Gao, RN; Wang, RX; Zhang, T; Ding, H; Li, B; Lu, CD; Shen, WD; Wei, ZG |
| 刊名 | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
 |
| 出版日期 | 2011 |
| 卷号 | 100期号:3页码:251-255 |
| 关键词 | Bombyx mori Cytochrome P950 genes Quantified transcription level Induced expression Xenobiotic metabolism |
| 通讯作者 | Shen, WD (reprint author), Soochow Univ, Sch Biol & Basic Med Sci, 199 Renai Rd,Dushu Lake Higher Educ Town,Suzhou I, Suzhou 215123, Jiangsu, Peoples R China.,sdgdzhao@163.com ; szwei2002@126.com |
| 英文摘要 | Cytochrome P450 (CYP) is a multifunctional super gene family, some of which play an important role in pesticide resistance. In this research, we used a real-time quantitative RT-PCR method, and a novel strategy, to measure the transcriptional level per gene copy using an exogenous RNA reference and DNA reference. The transcription levels of eight P450 genes in different tissues of fifth instar Bombyx mori larvae and their responses to pesticides (dichlorvos and deltamethrin) and sodium fluoride (NaF) were investigated. The results show different levels and patterns of expression of the different P450s in the various tissues observed. The P450s can be induced by pesticide and fluoride, but their responses to each are different. The results of this research are helpful in studying the tissue-specific expression of P450s in B. mori, and in developing new pesticide resistant silkworm varieties. (C) 2011 Elsevier Inc. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Entomology; Physiology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Entomology ; Physiology |
| 关键词[WOS] | GLUTATHIONE-S-TRANSFERASE ; MESSENGER-RNA ; MONOOXYGENASES ; EXPRESSION ; PCR |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000292575800008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/761]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhao, GD,Zhao, SS,Gao, RN,et al. Transcription profiling of eight cytochrome P450s potentially involved in xenobiotic metabolism in the silkworm, Bombyx mori[J]. PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY,2011,100(3):251-255. |
| APA | Zhao, GD.,Zhao, SS.,Gao, RN.,Wang, RX.,Zhang, T.,...&Wei, ZG.(2011).Transcription profiling of eight cytochrome P450s potentially involved in xenobiotic metabolism in the silkworm, Bombyx mori.PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY,100(3),251-255. |
| MLA | Zhao, GD,et al."Transcription profiling of eight cytochrome P450s potentially involved in xenobiotic metabolism in the silkworm, Bombyx mori".PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY 100.3(2011):251-255. |
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