Xeno-repopulation of Fah (-/-) Nod/Scid mice livers by human hepatocytes
文献类型：期刊论文
| 作者 | Su, BL; Liu, CC; Xiang, D; Zhang, HB; Yuan, SM; Wang, MJ; Chen, F; Zhu, HY; He, ZY; Wang, X |
| 刊名 | SCIENCE CHINA-LIFE SCIENCES
 |
| 出版日期 | 2011 |
| 卷号 | 54期号:3页码:227-234 |
| 关键词 | human hepatocyte humanized liver cell transplantation Fah gene knockout mice Nod/Scid mice |
| 通讯作者 | He, ZY (reprint author), Second Mil Med Univ, Dept Cell Biol, Shanghai 200433, Peoples R China.,zyhe@smmu.edu.cn ; yphu@smmu.edu.cn |
| 英文摘要 | Functional human hepatocytes xenografted into the liver of mice can be used as a model system to study pharmacokinetics, infection of hepatitis viruses, and the efficacy of hepatitis vaccines. Significant levels of liver xeno-repopulation have been reported in Fah (-/-) Rag2 (-/-) Il2rg (-/-) mice. However, the high mortality and low breeding rate of this model may hinder its application. A new model, termed Fah (-/-) Nod/Scid mice, which combines the advantages of liver repopulation in Fah (-/-) mice with the ease of xenotransplantation in Nod/Scid mice was obtained by gradual cross-breeding. Fah (-/-) Nod/Scid mice were easily maintained in breeding colonies and in adult animal care facilities. FK506 treatment combined with gradual withdrawal of NTBC before cell transplantation ensured that Fah (-/-) Nod/Scid mice were susceptible to liver xeno-repopulation by human hepatocytes; the proportion of engrafted human hepatocytes reached 33.6%. The function of the expanded human hepatocytes within the chimeric liver was confirmed by weight curve analysis, the expression of characteristic proteins, and the biochemical analysis of liver function. These results show that Fah (-/-) Nod/Scid mice are an ideal humanized liver mouse model with many useful applications. |
| 学科主题 | Life Sciences & Biomedicine - Other Topics |
| 类目[WOS] | Biology |
| 关键词[WOS] | HEPATITIS-B-VIRUS ; MOUSE-LIVER ; TRANSPLANTATION ; INFECTION ; ADULT ; FK506 ; RATS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000288507900004 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/764]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Su, BL,Liu, CC,Xiang, D,et al. Xeno-repopulation of Fah (-/-) Nod/Scid mice livers by human hepatocytes[J]. SCIENCE CHINA-LIFE SCIENCES,2011,54(3):227-234. |
| APA | Su, BL.,Liu, CC.,Xiang, D.,Zhang, HB.,Yuan, SM.,...&Hu, YP.(2011).Xeno-repopulation of Fah (-/-) Nod/Scid mice livers by human hepatocytes.SCIENCE CHINA-LIFE SCIENCES,54(3),227-234. |
| MLA | Su, BL,et al."Xeno-repopulation of Fah (-/-) Nod/Scid mice livers by human hepatocytes".SCIENCE CHINA-LIFE SCIENCES 54.3(2011):227-234. |
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