Establishment and characterization of primary lung cancer cell lines from Chinese population
文献类型：期刊论文
| 作者 | Zheng, C; Sun, YH; Ye, XL; Chen, HQ; Ji, HB |
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2011 |
| 卷号 | 32期号:3页码:385-392 |
| 关键词 | lung cancer primary cell lines epidermal growth factor receptor small cell lung carcinomas non-small cell lung carcinomas |
| 通讯作者 | Chen, HQ (reprint author), Fudan Univ, Dept Thorac Surg, Shanghai Canc Ctr, Dept Oncol,Shanghai Med Coll, Shanghai 200032, Peoples R China.,hqchen1@yahoo.com ; hbji@sibs.ac.cn |
| 英文摘要 | Aim: To establish and characterize primary lung cancer cell lines from Chinese population. Methods: Lung cancer specimens or pleural effusions were collected from Chinese lung cancer patients and cultured in vitro with ACL4 medium (for non-small cell lung carcinomas (NSCLC)) or HITES medium (for small cell lung carcinomas (SCLC)) supplemented with 5% FBS. All cell lines were maintained in culture for more than 25 passages. Most of these cell lines were further analyzed for oncogenic mutations, karyotype, cell growth kinetics, and tumorigenicity in nude mice. Results: Eight primary cell lines from Chinese lung cancer patients were established and characterized, including seven NSCLC cell lines and one SCLC cell line. Five NSCLC cell lines were found to harbor epidermal growth factor receptor (EGFR) kinase domain mutations. Conclusion: These well-characterized primary lung cancer cell lines from Chinese population provide a unique platform for future studies of the ethnic differences in lung cancer biology and drug response. |
| 学科主题 | Chemistry; Pharmacology & Pharmacy |
| 类目[WOS] | Chemistry, Multidisciplinary ; Pharmacology & Pharmacy |
| 关键词[WOS] | GROWTH-FACTOR RECEPTOR ; TYROSINE KINASE INHIBITORS ; FREE DEFINED MEDIUM ; KRAS MUTATIONS ; NEVER-SMOKERS ; ETHNIC-DIFFERENCES ; CULTURE METHODS ; EGFR MUTATIONS ; CARCINOMA ; ADENOCARCINOMAS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000288003200015 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/765]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zheng, C,Sun, YH,Ye, XL,et al. Establishment and characterization of primary lung cancer cell lines from Chinese population[J]. ACTA PHARMACOLOGICA SINICA,2011,32(3):385-392. |
| APA | Zheng, C,Sun, YH,Ye, XL,Chen, HQ,&Ji, HB.(2011).Establishment and characterization of primary lung cancer cell lines from Chinese population.ACTA PHARMACOLOGICA SINICA,32(3),385-392. |
| MLA | Zheng, C,et al."Establishment and characterization of primary lung cancer cell lines from Chinese population".ACTA PHARMACOLOGICA SINICA 32.3(2011):385-392. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。