Activity of superior interferon alpha against HIV-1 in severe combined immunodeficient mice reconstituted with human peripheral blood leukocytes
文献类型：期刊论文
| 作者 | Zhang, W; Tong, XA; Nakasone, T; Yue, XT; Yamamoto, N; Liu, XY; Yang, RG |
| 刊名 | CHINESE MEDICAL JOURNAL
 |
| 出版日期 | 2011 |
| 卷号 | 124期号:3页码:396-400 |
| 关键词 | human immunodeficiency virus type 1 severe combined immunodeficient mouse interferon-alpha |
| 通讯作者 | Yang, RG (reprint author), Chinese Acad Sci, Wuhan Inst Virol, Lab HIV Mol Epidemiol & Virol, State Key Lab Virol, Wuhan 430071, Hubei, Peoples R China.,ryang@wh.iov.cn |
| 英文摘要 | Background Interferon (IFN) can inhibit human immunodeficiency virus type 1 (HIV-1) replication in vitro and in clinic. However, IFN therapy for HIV infection was limited by its moderate antiviral efficacy and its frequent adverse effects. In the present study we evaluated the anti-HIV efficacy of a novel synthesized superior interferon alpha (sIFN alpha). Methods We performed in vitro experiments with HIV-1 IIIB infected MT4 cells, and evaluated in vivo anti-HIV efficacy of sIFN alpha in severe combined immunodeficient (SCID) mice reconstituted with human peripheral blood leukocytes (hu-PBL-SCID mice). Results We found that the 50% effective concentrations (EC50) of sIFN alpha against the replication of HIV-1 in MT4 cells was 0.06 ng/ml, representing stronger antiviral activity than interferon-alpha in vitro. In the hu-PBL-SCID mice, a dose-dependent protection pattern was observed: with 0.45 mu g and 1.35 mu g sIFN alpha daily treatments, parts of SCID mice were protected from HIV infection, whereas 2.25 mu g sIFN alpha daily treatments resulted in a terminally complete protection. Conclusions sIFN alpha shows good anti-HIV activity both in vitro and in SCID mice, may be a promising anti-HIV agent deserving clinical investigation, especially considering the potential of IFN-alpha to inhibit HIV replication in patients infected with drug-resistant variants or co-infected with hepatitis C virus (HCV). Chin Med J 2011;124(3):396-400 |
| 学科主题 | General & Internal Medicine |
| 类目[WOS] | Medicine, General & Internal |
| 关键词[WOS] | VIRUS TYPE-1 INFECTION ; HEPATITIS-C VIRUS ; PBL-SCID MICE ; INJECTION-DRUG USERS ; REPLICATION INVITRO ; I INTERFERON ; HU MICE ; CHINA ; AIDS ; COINFECTION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000287992400014 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/767]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, W,Tong, XA,Nakasone, T,et al. Activity of superior interferon alpha against HIV-1 in severe combined immunodeficient mice reconstituted with human peripheral blood leukocytes[J]. CHINESE MEDICAL JOURNAL,2011,124(3):396-400. |
| APA | Zhang, W.,Tong, XA.,Nakasone, T.,Yue, XT.,Yamamoto, N.,...&Yang, RG.(2011).Activity of superior interferon alpha against HIV-1 in severe combined immunodeficient mice reconstituted with human peripheral blood leukocytes.CHINESE MEDICAL JOURNAL,124(3),396-400. |
| MLA | Zhang, W,et al."Activity of superior interferon alpha against HIV-1 in severe combined immunodeficient mice reconstituted with human peripheral blood leukocytes".CHINESE MEDICAL JOURNAL 124.3(2011):396-400. |
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