Impact of intra-subunit interactions on the dimeric arginine kinase activity and structural stability
文献类型：期刊论文
| 作者 | Wu, QY; Jin, KZ; Li, F; Hu, ZQ; Wang, XY |
| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| 出版日期 | 2011 |
| 卷号 | 49期号:4页码:822-831 |
| 关键词 | Arginine kinase Aggregation Conformational change Domain-domain interactions Structural stability |
| 通讯作者 | Hu, ZQ (reprint author), Second Mil Med Univ, Shanghai Chang Zheng Hosp, Dept Gen Surg, Shanghai, Peoples R China.,huzq62@yahoo.cn ; xyunwang@sdau.edu.cn |
| 英文摘要 | Arginine kinase (AK) catalyzes the reversible phosphorylation of arginine by ATP, yielding the phosphoarginine. In this research, six conserved residues located on the intra-subunit domain-domain interfaces were mutated to explore their roles in the activity and structural stability of dimer AK. The mutations D69A, E70A, E71A and F80A led to pronounced loss of AK activity and structural stability. Although the mutations V75A and F76A had little effect on AK activity and structure, they caused gradually decreased the stability and reactivation of dimer AK. Our results suggested that the mutations might affect the correct positioning of the N-loop and C-loop thus disrupted the efficient recognition and interactions between the N-terminal domain and C-terminal domain which may influence the compact dimer structure, and result in decreased activity and structural stability. (C) 2011 Elsevier B.V. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | MUSCLE CREATINE-KINASE ; CUCUMBER STICHOPUS-JAPONICUS ; AMINO-ACID-RESIDUES ; SITE ; INACTIVATION ; PURIFICATION ; SYNERGISM ; BINDING ; LOCUST |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000295502500055 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/769]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wu, QY,Jin, KZ,Li, F,et al. Impact of intra-subunit interactions on the dimeric arginine kinase activity and structural stability[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2011,49(4):822-831. |
| APA | Wu, QY,Jin, KZ,Li, F,Hu, ZQ,&Wang, XY.(2011).Impact of intra-subunit interactions on the dimeric arginine kinase activity and structural stability.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,49(4),822-831. |
| MLA | Wu, QY,et al."Impact of intra-subunit interactions on the dimeric arginine kinase activity and structural stability".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 49.4(2011):822-831. |
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