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Genome-wide Regulation of 5hmC, 5mC, and Gene Expression by Tet1 Hydroxylase in Mouse Embryonic Stem Cells
文献类型：期刊论文
| 作者 | Xu, YF; Wu, FZ; Tan, L; Kong, LC; Xiong, LJ; Deng, J; Barbera, AJ; Zheng, LJ; Zhang, HK; Huang, S |
| 刊名 | MOLECULAR CELL
 |
| 出版日期 | 2011 |
| 卷号 | 42期号:4页码:451-464 |
| 通讯作者 | Shi, YG (reprint author), Fudan Univ, Lab Epigenet, Inst Biomed Sci, Shanghai 200032, Peoples R China.,yujiang_shi@hms.harvard.edu |
| 英文摘要 | DNA methylation at the 5 position of cytosine (5mC) in the mammalian genome is a key epigenetic event critical for various cellular processes. The ten-eleven translocation (Tet) family of 5mC-hydroxylases, which convert 5mC to 5-hydroxymethylcytosine (5hmC), offers a way for dynamic regulation of DNA methylation. Here we report that Tet1 binds to unmodified C or 5mC- or 5hmC-modified CpG-rich DNA through its CXXC domain. Genome-wide mapping of Tet1 and 5hmC reveals mechanisms by which Tet1 controls 5hmC and 5mC levels in mouse embryonic stem cells (mESCs). We also uncover a comprehensive gene network influenced by Tet1. Collectively, our data suggest that Tet1 controls DNA methylation both by binding to CpG-rich regions to prevent unwanted DNA methyltransferase activity, and by converting 5mC to 5hmC through hydroxylase activity. This Tet1-mediated antagonism of CpG methylation imparts differential maintenance of DNA methylation status at Tet1 targets, ultimately contributing to mESC differentiation and the onset of embryonic development. |
| 学科主题 | Biochemistry & Molecular Biology; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 关键词[WOS] | DNA METHYLATION ; RNA-SEQ ; SELF-RENEWAL ; 5-HYDROXYMETHYLCYTOSINE ; 5-METHYLCYTOSINE ; BINDING ; DIFFERENTIATION ; QUANTIFICATION ; SPECIFICATION ; TRANSCRIPTION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000291113800008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/772]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Xu, YF,Wu, FZ,Tan, L,et al. Genome-wide Regulation of 5hmC, 5mC, and Gene Expression by Tet1 Hydroxylase in Mouse Embryonic Stem Cells[J]. MOLECULAR CELL,2011,42(4):451-464. |
| APA | Xu, YF.,Wu, FZ.,Tan, L.,Kong, LC.,Xiong, LJ.,...&Shi, YG.(2011).Genome-wide Regulation of 5hmC, 5mC, and Gene Expression by Tet1 Hydroxylase in Mouse Embryonic Stem Cells.MOLECULAR CELL,42(4),451-464. |
| MLA | Xu, YF,et al."Genome-wide Regulation of 5hmC, 5mC, and Gene Expression by Tet1 Hydroxylase in Mouse Embryonic Stem Cells".MOLECULAR CELL 42.4(2011):451-464. |
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