Defects in Trophoblast Cell Lineage Account for the Impaired In Vivo Development of Cloned Embryos Generated by Somatic Nuclear Transfer
文献类型：期刊论文
| 作者 | Lin, JW; Shi, LY; Zhang, M; Yang, H; Qin, YR; Zhang, J; Gong, DQ; Zhang, X; Li, DS; Li, JS |
| 刊名 | CELL STEM CELL
 |
| 出版日期 | 2011 |
| 卷号 | 8期号:4页码:371-375 |
| 通讯作者 | Li, JS (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,jsli@sibs.ac.cn |
| 英文摘要 | The low success rate of somatic nuclear transfer (NT) is hypothesized to be mainly due to functional defects in the trophoblast cell lineage rather than the inner cell mass (ICM); this hypothesis, however, remains to be tested directly. Here we separated the ICMs from cloned blastocysts and aggregated the cloned ICM with two fertilization-derived (FD) tetraploid (4N) embryos. We found that the full-term development of cloned ICMs was dramatically improved after the trophoblast cells in the cloned blastocysts were replaced by cells from tetraploid embryos, thus providing direct evidence that defects in trophoblast cell lineage underlie the low success rate of somatic NT. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell & Tissue Engineering ; Cell Biology |
| 关键词[WOS] | STEM-CELLS ; ES CELLS ; MOUSE BLASTOCYSTS ; TETRAPLOID CELLS ; MICE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000289707100009 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/774]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Lin, JW,Shi, LY,Zhang, M,et al. Defects in Trophoblast Cell Lineage Account for the Impaired In Vivo Development of Cloned Embryos Generated by Somatic Nuclear Transfer[J]. CELL STEM CELL,2011,8(4):371-375. |
| APA | Lin, JW.,Shi, LY.,Zhang, M.,Yang, H.,Qin, YR.,...&Li, JS.(2011).Defects in Trophoblast Cell Lineage Account for the Impaired In Vivo Development of Cloned Embryos Generated by Somatic Nuclear Transfer.CELL STEM CELL,8(4),371-375. |
| MLA | Lin, JW,et al."Defects in Trophoblast Cell Lineage Account for the Impaired In Vivo Development of Cloned Embryos Generated by Somatic Nuclear Transfer".CELL STEM CELL 8.4(2011):371-375. |
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