Human amniotic epithelial cells maintain mouse spermatogonial stem cells in an undifferentiated state due to high leukemia inhibitor factor (LIF) expression
文献类型：期刊论文
| 作者 | Liu, T; Guo, LH; Liu, ZX; Cheng, WW |
| 刊名 | IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
 |
| 出版日期 | 2011 |
| 卷号 | 47期号:4页码:318-326 |
| 关键词 | Leukemia inhibitor factor Human amnion epithelial cells Mouse spermatogonial stem cells Undifferentiated Self-renewal |
| 通讯作者 | Liu, ZX (reprint author), Tongji Univ, Sch Life Sci & Technol, Shanghai 200092, Peoples R China.,liute79@yahoo.com ; liute79@yahoo.com |
| 英文摘要 | Spermatogonial stem cells (SSCs), like other stem cells, have unique properties: prolonged proliferation, self-renewal, generation of differentiated progeny, and maintenance of developmental potential. Long-term cultivation of normal SSCs into stable cell lines, and maintaining SSCs in an undifferentiated state capable of self-renewal, is a major challenge. Here, we compare the effect of leukemia inhibitory factor (LIF) expression on mouse SSCs isolated from testicular tissue cultured under different conditions. We found that human amniotic epithelial cells (hAECs) with high LIF expression (LIF (high) ) feeder cells allowed mouse SSCs to maintain a high level of AP activity when cultured long term. Expression of some important stem cell markers was higher in mouse SSCs cultured on hAECs (LIF (high) ) compared to those cultured on hAECs (LIF (low) ). Taken together, these results suggest that LIF expression could be a crucial component for feeder cells to maintain mouse SSCs in an undifferentiated, proliferative state capable of self-renewal. |
| 学科主题 | Cell Biology; Developmental Biology |
| 类目[WOS] | Cell Biology ; Developmental Biology |
| 关键词[WOS] | SELF-RENEWAL ; SIGNALING MAINTAINS ; HUMAN TESTIS ; GENERATION ; DIFFERENTIATION ; TRANSPLANTATION ; IDENTIFICATION ; PROLIFERATION ; PLURIPOTENCY ; MAINTENANCE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000289900700009 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/775]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Liu, T,Guo, LH,Liu, ZX,et al. Human amniotic epithelial cells maintain mouse spermatogonial stem cells in an undifferentiated state due to high leukemia inhibitor factor (LIF) expression[J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL,2011,47(4):318-326. |
| APA | Liu, T,Guo, LH,Liu, ZX,&Cheng, WW.(2011).Human amniotic epithelial cells maintain mouse spermatogonial stem cells in an undifferentiated state due to high leukemia inhibitor factor (LIF) expression.IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL,47(4),318-326. |
| MLA | Liu, T,et al."Human amniotic epithelial cells maintain mouse spermatogonial stem cells in an undifferentiated state due to high leukemia inhibitor factor (LIF) expression".IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL 47.4(2011):318-326. |
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