Reprogramming of ovine adult fibroblasts to pluripotency via drug-inducible expression of defined factors
文献类型：期刊论文
| 作者 | Bao, L; He, LXZ; Chen, JJ; Wu, Z; Liao, J; Rao, LJ; Ren, JT; Li, H; Zhu, H; Qian, L |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2011 |
| 卷号 | 21期号:4页码:600-608 |
| 关键词 | sheep pluripotency reprogram iPS cells embryonic stem cells |
| 通讯作者 | Xiao, L (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, Shanghai 200031, Peoples R China.,leixiao@sibs.ac.cn |
| 英文摘要 | Reprogramming of somatic cells in the enucleated egg made Dolly, the sheep, the first successfully cloned mammal in 1996. However, the mechanism of sheep somatic cell reprogramming has not yet been addressed. Moreover, sheep embryonic stem (ES) cells are still not available, which limits the generation of precise gene-modified sheep. In this study, we report that sheep somatic cells can be directly reprogrammed to induced pluripotent stem (iPS) cells using defined factors (Oct4, Sox2, c-Myc, Klf4, Nanog, Lin28, SV40 large T and hTERT). Our observations indicated that somatic cells from sheep are more difficult to reprogram than somatic cells from other species, in which iPS cells have been reported. We demonstrated that sheep iPS cells express ES cell markers, including alkaline phosphatase, Oct4, Nanog, Sox2, Rex1, stage-specific embryonic antigen-1, TRA-1-60, TRA-1-81 and E-cadherin. Sheep iPS cells exhibited normal karyotypes and were able to differentiate into all three germ layers both in vitro and in teratomas. Our study may help to reveal the mechanism of somatic cell reprogramming in sheep and provide a platform to explore the culture conditions for sheep ES cells. Moreover, sheep iPS cells may be directly used to generate precise gene-modified sheep. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | STEM-CELL LINES ; HUMAN SOMATIC-CELLS ; GROWTH-FACTOR ; TRANSGENIC SHEEP ; IPS CELLS ; RAT BLASTOCYSTS ; MOUSE EMBRYOS ; IN-VITRO ; GENERATION ; PIG |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000289230800007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/779]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Bao, L,He, LXZ,Chen, JJ,et al. Reprogramming of ovine adult fibroblasts to pluripotency via drug-inducible expression of defined factors[J]. CELL RESEARCH,2011,21(4):600-608. |
| APA | Bao, L.,He, LXZ.,Chen, JJ.,Wu, Z.,Liao, J.,...&Xiao, L.(2011).Reprogramming of ovine adult fibroblasts to pluripotency via drug-inducible expression of defined factors.CELL RESEARCH,21(4),600-608. |
| MLA | Bao, L,et al."Reprogramming of ovine adult fibroblasts to pluripotency via drug-inducible expression of defined factors".CELL RESEARCH 21.4(2011):600-608. |
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