Reprogramming of mouse and human somatic cells by high-performance engineered factors
文献类型：期刊论文
| 作者 | Wang, Y; Chen, JK; Hu, JL; Wei, XX; Qin, DJ; Gao, JA; Zhang, L; Jiang, J; Li, JS; Liu, J |
| 刊名 | EMBO REPORTS
 |
| 出版日期 | 2011 |
| 卷号 | 12期号:4页码:373-378 |
| 关键词 | reprogramming factor iPS induced pluripotency reprogramming stem cells |
| 通讯作者 | Pei, DQ (reprint author), Chinese Acad Sci, Guangzhou Inst Biomed, S China Inst Stem Cell Biol & Regenerat Med, Key Lab Regenerat Biol, Guangzhou 510663, Guangdong, Peoples R China.,pei_duanqing@gibh.ac.cn ; glxu@sibs.ac.cn |
| 英文摘要 | Reprogramming somatic cells to become induced pluripotent stem cells (iPSCs) by using defined factors represents an important breakthrough in biology and medicine, yet remains inefficient and poorly understood. We therefore devised synthetic factors by fusing the VP16 transactivation domain to OCT4 (also known as Pou5f1), NANOG and SOX2, respectively. These synthetic factors could reprogramme both mouse and human fibroblasts with enhanced efficiency and accelerated kinetics. Remarkably, Oct4-VP16 alone could efficiently reprogramme mouse embryonic fibroblasts (MEFs) into germline-competent iPSCs. Furthermore, episomally delivered synthetic factors could reproducibly generate integration-free iPSCs from MEFs with enhanced efficiency. Our results not only demonstrate the feasibility of engineering more potent reprogramming factors, but also suggest that transcriptional reactivation of OCT4 target genes might be a rate-limiting step in the conversion of somatic cells to pluripotent cells. Synthetic factor-based reprogramming might lead to a paradigm shift in reprogramming research. |
| 学科主题 | Biochemistry & Molecular Biology; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 关键词[WOS] | PLURIPOTENT STEM-CELLS ; ACTIVATION DOMAIN ; IPS CELLS ; GENERATION ; TRANSCRIPTION ; VP16 ; FIBROBLASTS ; INDUCTION ; PATHWAY ; LINKING |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000289073200018 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/782]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, Y,Chen, JK,Hu, JL,et al. Reprogramming of mouse and human somatic cells by high-performance engineered factors[J]. EMBO REPORTS,2011,12(4):373-378. |
| APA | Wang, Y.,Chen, JK.,Hu, JL.,Wei, XX.,Qin, DJ.,...&Xu, GL.(2011).Reprogramming of mouse and human somatic cells by high-performance engineered factors.EMBO REPORTS,12(4),373-378. |
| MLA | Wang, Y,et al."Reprogramming of mouse and human somatic cells by high-performance engineered factors".EMBO REPORTS 12.4(2011):373-378. |
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