A high-quality secretome of A549 cells aided the discovery of C4b-binding protein as a novel serum biomarker for non-small cell lung cancer
文献类型：期刊论文
| 作者 | Luo, XY; Liu, YS; Wang, R; Hu, HC; Zeng, R; Chen, HQ |
| 刊名 | JOURNAL OF PROTEOMICS
 |
| 出版日期 | 2011 |
| 卷号 | 74期号:4页码:528-538 |
| 关键词 | Non-small cell lung cancer Cancer secretome Biomarkers Proteomics |
| 通讯作者 | Zeng, R (reprint author), 320 YueYang Rd, Shanghai 200031, Peoples R China.,zr@sibs.ac.cn ; chenhq@shca.org.cn |
| 英文摘要 | Cancer secretomes are a promising source for biomarker discovery. The analysis of cancer secretomes still faces some difficulties mainly related to the intracellular contamination, which hinders the qualification and follow-up validations. This study aimed to establish a high-quality secretome of A549 cells by using the cellular proteome as a reference and to test the merits of this refined secretome for biomarker discovery for non-small cell lung cancer (NSCLC). Using one-dimensional gel electrophoresis followed by liquid-chromatography tandem mass spectrometry, we comprehensively investigated the secretome and the concurrent cellular proteome of A549 cells. A high-quality secretome consisting of 382 proteins was refined from 889 initial secretory proteins. More than 85.3% of proteins were annotated as secreted and 76.8% as extracellular or membrane-bound. The discriminative power of the lung-cancer associated secretome was confirmed by gene expression and serum proteomic data. The elevated level of C4b-binding Protein (C4BP) in NSCLC blood was verified by enzyme-linked immunosorbent assays (ELISA, p = 6.07e-6). Moreover, the serum C4BP level in 89 patients showed a strong association with the clinical staging of NSCLC. Our reference-experiment-driven strategy is simple and widely applicable, and may facilitate the identification of novel promising biomarkers of lung cancer. (C) 2011 Elsevier B.V. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemical Research Methods |
| 关键词[WOS] | CONDITIONED MEDIA ; PROTEOMICS ANALYSIS ; HUMAN BLOOD ; LABEL-FREE ; LINES ; IDENTIFICATION ; PLASMA ; PHOSPHOPROTEOME ; ADENOCARCINOMA ; ANGIOGENESIS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000289022900014 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/783]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Luo, XY,Liu, YS,Wang, R,et al. A high-quality secretome of A549 cells aided the discovery of C4b-binding protein as a novel serum biomarker for non-small cell lung cancer[J]. JOURNAL OF PROTEOMICS,2011,74(4):528-538. |
| APA | Luo, XY,Liu, YS,Wang, R,Hu, HC,Zeng, R,&Chen, HQ.(2011).A high-quality secretome of A549 cells aided the discovery of C4b-binding protein as a novel serum biomarker for non-small cell lung cancer.JOURNAL OF PROTEOMICS,74(4),528-538. |
| MLA | Luo, XY,et al."A high-quality secretome of A549 cells aided the discovery of C4b-binding protein as a novel serum biomarker for non-small cell lung cancer".JOURNAL OF PROTEOMICS 74.4(2011):528-538. |
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