The epididymis-specific antimicrobial peptide beta-defensin 15 is required for sperm motility and male fertility in the rat (Rattus norvegicus)
文献类型：期刊论文
| 作者 | Zhao, Y; Diao, H; Ni, ZM; Hu, SG; Yu, HG; Zhang, YL |
| 刊名 | CELLULAR AND MOLECULAR LIFE SCIENCES
 |
| 出版日期 | 2011 |
| 卷号 | 68期号:4页码:697-708 |
| 关键词 | beta-Defensin Epididymis Sperm maturation Sperm motility Male fertility Antimicrobial activity |
| 通讯作者 | Zhang, YL (reprint author), Chinese Acad Sci, Shanghai Key Lab Mol Androl, State Key Lab Mol Biol, Inst Biochem & Cell Biol,Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,ylzhang@sibs.ac.cn |
| 英文摘要 | Spermatozoa acquire forward motility and fertilizing capacity during their transit through the epididymis. The maturation process involves modifications of the sperm surface by different proteins that are secreted by a series of specialized regions in the epididymal epithelium. Here we show that the rat epididymis-specific beta-defensin 15 (Defb15) exhibits an androgen-dependent expression pattern, and it can bind to the acrosomal region of caput sperm. Coculture of caput spermatozoa with Defb15 antibody in vitro resulted in a significant decline in sperm motility. Moreover, the total and progressive motility of the spermatozoa dramatically decreased in rats when Defb15 was downregulated by lentivirus-mediated RNAi in vivo. Remarkably, knock down of Defb15 led to a reduction in fertility and embryonic development failure. In addition, the recombinant Defb15 showed antimicrobial activity in a dose-dependent fashion. These results suggest that Defb15 plays a dual role in both sperm maturation and pathogen defense in rat epididymis. |
| 学科主题 | Biochemistry & Molecular Biology; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 关键词[WOS] | MALE REPRODUCTIVE-TRACT ; IN-VITRO FERTILIZATION ; ESCHERICHIA-COLI ; BINDING-PROTEIN ; GENE CLUSTERS ; EXPRESSION ; CAPACITATION ; SPERMATOZOA ; MATURATION ; FAMILY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000286662700011 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/787]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhao, Y,Diao, H,Ni, ZM,et al. The epididymis-specific antimicrobial peptide beta-defensin 15 is required for sperm motility and male fertility in the rat (Rattus norvegicus)[J]. CELLULAR AND MOLECULAR LIFE SCIENCES,2011,68(4):697-708. |
| APA | Zhao, Y,Diao, H,Ni, ZM,Hu, SG,Yu, HG,&Zhang, YL.(2011).The epididymis-specific antimicrobial peptide beta-defensin 15 is required for sperm motility and male fertility in the rat (Rattus norvegicus).CELLULAR AND MOLECULAR LIFE SCIENCES,68(4),697-708. |
| MLA | Zhao, Y,et al."The epididymis-specific antimicrobial peptide beta-defensin 15 is required for sperm motility and male fertility in the rat (Rattus norvegicus)".CELLULAR AND MOLECULAR LIFE SCIENCES 68.4(2011):697-708. |
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