Co-Chaperone HSJ1a Dually Regulates the Proteasomal Degradation of Ataxin-3
文献类型：期刊论文
| 作者 | Gao, XC; Zhou, CJ; Zhou, ZR; Zhang, YH; Zheng, XM; Song, AX; Hu, HY |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2011 |
| 卷号 | 6期号:5页码:e19763-e19763 |
| 通讯作者 | Gao, XC (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai, Peoples R China.,hyhu@sibs.ac.cn |
| 英文摘要 | Homo sapiens J domain protein (HSJ1) is a J-domain containing co-chaperone that is known to stimulate ATPase activity of HSP70 chaperone, while it also harbors two ubiquitin (Ub)-interacting motifs (UIMs) that may bind with ubiquitinated substrates and potentially function in protein degradation. We studied the effects of HSJ1a on the protein levels of both normal and the disease-related polyQ-expanded forms of ataxin-3 (Atx3) in cells. The results demonstrate that the N-terminal J-domain and the C-terminal UIM domain of HSJ1a exert opposite functions in regulating the protein level of cellular overexpressed Atx3. This dual regulation is dependent on the binding of the J-domain with HSP70, and the UIM domain with polyUb chains. The J-domain down-regulates the protein level of Atx3 through HSP70 mediated proteasomal degradation, while the UIM domain may alleviate this process via maintaining the ubiquitinated Atx3. We propose that cochaperone HSJ1a orchestrates the balance of substrates in stressed cells in a Yin-Yang manner. |
| 学科主题 | Science & Technology - Other Topics |
| 类目[WOS] | Multidisciplinary Sciences |
| 关键词[WOS] | HEAT-SHOCK-PROTEIN ; UBIQUITIN-DEPENDENT DEGRADATION ; MACHADO-JOSEPH-DISEASE ; NITRIC-OXIDE SYNTHASE ; HSP70 ATPASE ACTIVITY ; MOLECULAR CHAPERONES ; POLYGLUTAMINE DISEASE ; MISFOLDING DISEASES ; SUBSTRATE-BINDING ; QUALITY-CONTROL |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000290771200020 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/793]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Gao, XC,Zhou, CJ,Zhou, ZR,et al. Co-Chaperone HSJ1a Dually Regulates the Proteasomal Degradation of Ataxin-3[J]. PLOS ONE,2011,6(5):e19763-e19763. |
| APA | Gao, XC.,Zhou, CJ.,Zhou, ZR.,Zhang, YH.,Zheng, XM.,...&Hu, HY.(2011).Co-Chaperone HSJ1a Dually Regulates the Proteasomal Degradation of Ataxin-3.PLOS ONE,6(5),e19763-e19763. |
| MLA | Gao, XC,et al."Co-Chaperone HSJ1a Dually Regulates the Proteasomal Degradation of Ataxin-3".PLOS ONE 6.5(2011):e19763-e19763. |
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