Quantitative detection of single amino acid polymorphisms by targeted proteomics
文献类型：期刊论文
| 作者 | Su, ZD; Sun, L; Yu, DX; Li, RX; Li, HX; Yu, ZJ; Sheng, QH; Lin, X; Zeng, R; Wu, JR |
| 刊名 | JOURNAL OF MOLECULAR CELL BIOLOGY
 |
| 出版日期 | 2011 |
| 卷号 | 3期号:5页码:309-315 |
| 关键词 | single amino acid polymorphism (SAP) proteomics selected reaction monitoring (SRM) diabetes obesity |
| 通讯作者 | Zeng, R (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Key Lab Syst Biol, Shanghai 200031, Peoples R China.,zr@sibs.ac.cn ; wujr@sibs.ac.cn |
| 英文摘要 | Single-nucleotide polymorphisms (SNPs) are recognized as one kind of major genetic variants in population scale. However, polymorphisms at the proteome level in population scale remain elusive. In the present study, we named amino acid variances derived from SNPs within coding regions as single amino acid polymorphisms (SAPs) at the proteome level, and developed a pipeline of non-targeted and targeted proteomics to identify and quantify SAP peptides in human plasma. The absolute concentrations of three selected SAP-peptide pairs among 290 Asian individuals were measured by selected reaction monitoring (SRM) approach, and their associations with both obesity and diabetes were further analyzed. This work revealed that heterozygotes and homozygotes with various SAPs in a population could have different associations with particular traits. In addition, the SRM approach allows us for the first time to separately measure the absolute concentration of each SAP peptide in the heterozygotes, which also shows different associations with particular traits. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | GENE ; EXPRESSION ; HAPLOTYPE ; PROTEINS ; PLASMA ; SERUM |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000296292900008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/798]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Su, ZD,Sun, L,Yu, DX,et al. Quantitative detection of single amino acid polymorphisms by targeted proteomics[J]. JOURNAL OF MOLECULAR CELL BIOLOGY,2011,3(5):309-315. |
| APA | Su, ZD.,Sun, L.,Yu, DX.,Li, RX.,Li, HX.,...&Wu, JR.(2011).Quantitative detection of single amino acid polymorphisms by targeted proteomics.JOURNAL OF MOLECULAR CELL BIOLOGY,3(5),309-315. |
| MLA | Su, ZD,et al."Quantitative detection of single amino acid polymorphisms by targeted proteomics".JOURNAL OF MOLECULAR CELL BIOLOGY 3.5(2011):309-315. |
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