Lyzl4, a novel mouse sperm-related protein, is involved in fertilization
文献类型：期刊论文
| 作者 | Sun, RL; Shen, RL; Li, J; Xu, GJ; Chi, J; Li, LM; Ren, JK; Wang, ZG; Fei, J |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2011 |
| 卷号 | 43期号:5页码:346-353 |
| 关键词 | Lyzl4 spermatozoa fertilization gene cloning |
| 通讯作者 | Fei, J (reprint author), Shanghai Res Ctr Model Organisms, Shanghai 201210, Peoples R China.,jfei@tongji.edu.cn |
| 英文摘要 | The role of Chicken-type (c-type) lysozyme, a prototype lysozyme, in immunity has been characterized in many organisms. In this study, we cloned a novel c-type lysozyme-like gene, Lyzl4, which was located on mouse chromosome 9F4 and encoded 145 amino acids with a putative signal peptide and a protease cleavage site. The mature recombinant Lyzl4 protein expressed in yeast did not show the bacteriolytic activity. Sequence alignment analysis demonstrated that 3 of the 20 invariant residues in c-type lysozymes were changed in Lyzl4. One of the 'changed' amino acids (D52G) is located in the catalytic domain. Lyzl4 mRNA was selectively expressed in testis and epididymis in adult mice, with varying expression level across different developmental stages. High level of Lyzl4 protein was found on the spermatozoa of acrosomal region and principal piece of tail. Immuno-neutralization of Lyzl4 protein in spermatozoa with its specific antibody significantly decreased in vitro fertilization percentage in a dose-dependent manner, suggesting that Lyzl4 might be important for fertilization. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | LYSOZYME-LIKE PROTEIN ; ALPHA-LACTALBUMIN ; MURAMIDASE ; SYSTEM ; FAMILY ; SLLP1 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000289836200003 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/809]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Sun, RL,Shen, RL,Li, J,et al. Lyzl4, a novel mouse sperm-related protein, is involved in fertilization[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2011,43(5):346-353. |
| APA | Sun, RL.,Shen, RL.,Li, J.,Xu, GJ.,Chi, J.,...&Fei, J.(2011).Lyzl4, a novel mouse sperm-related protein, is involved in fertilization.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,43(5),346-353. |
| MLA | Sun, RL,et al."Lyzl4, a novel mouse sperm-related protein, is involved in fertilization".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 43.5(2011):346-353. |
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