Effect of chymotrypsin C and related proteins on pancreatic cancer cell migration
文献类型：期刊论文
| 作者 | Wang, HB; Sha, W; Liu, ZX; Chi, CW |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2011 |
| 卷号 | 43期号:5页码:362-371 |
| 关键词 | chymotrypsin C cytokeratin 18 proteomics two-dimensional fluorescence differential in gel electrophoresis (2D-DIGE) cell migration |
| 通讯作者 | Chi, CW (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Key Lab Mol Biol, Shanghai 200031, Peoples R China.,zwqi@sibs.ac.cn |
| 英文摘要 | Pancreatic cancer is a malignant cancer with a high mortality rate. The amount of chymotrypsin C in pancreatic cancer cells is only 20% of that found in normal cells. Chymotrypsin C has been reported to be involved in cancer cell apoptosis, but its effect on pancreatic cancer cell migration is unclear. We performed cell migration scratch assays and Transwell experiments, and found that cell migration ability was downregulated in pancreatic cancer Aspc-1 cells that overexpressed chymotrypsin C, whereas the cell migration ability was upregulated in Aspc-1 cells in which chymotrypsin C was suppressed. Two-dimensional fluorescence differential in gel electrophoresis/mass spectrometry method was used to identify the proteins that were differentially expressed in Aspc-1 cells that were transfected with plasmids to induce either overexpression or suppressed expression of chymotrypsin C. Among 26 identified differential proteins, cytokeratin 18 was most obviously correlated with chymotrypsin C expression. Cytokeratin 18 is expressed in developmental tissues in early stages of cancer, and is highly expressed in most carcinomas. We speculated that chymotrypsin C might regulate pancreatic cancer cell migration in relation to cytokeratin 18 expression. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | CYTOKERATIN 18 ; RT-PCR ; ENZYMATIC SPECIFICITY ; CARCINOMA-CELLS ; BONE-MARROW ; EXPRESSION ; ACTIVATION ; MICROMETASTASIS ; ADENOCARCINOMA ; POLYPEPTIDES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000289836200005 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/810]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, HB,Sha, W,Liu, ZX,et al. Effect of chymotrypsin C and related proteins on pancreatic cancer cell migration[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2011,43(5):362-371. |
| APA | Wang, HB,Sha, W,Liu, ZX,&Chi, CW.(2011).Effect of chymotrypsin C and related proteins on pancreatic cancer cell migration.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,43(5),362-371. |
| MLA | Wang, HB,et al."Effect of chymotrypsin C and related proteins on pancreatic cancer cell migration".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 43.5(2011):362-371. |
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