Sox31 is involved in central nervous system anteroposterior regionalization through regulating the organizer activity in zebrafish
文献类型：期刊论文
| 作者 | Hu, SN; Wu, ZL; Yan, YC; Li, YP |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2011 |
| 卷号 | 43期号:5页码:387-399 |
| 关键词 | Sox31 organizer specification central nervous system AP patterning mid-blastula transition |
| 通讯作者 | Li, YP (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Lab Mol Cell Biol,Shanghai Key Lab Mol Androl, Shanghai 200031, Peoples R China.,yipingli@sibs.ac.cn |
| 英文摘要 | Sox superfamily proteins are DNA-binding transcriptional factors that contain highly conserved high-mobility group (HMG) box and take part in various development process. Sox31 is a maternal factor supplied in the oocyte and starts its zygotic expression during mid-blastula transition (MBT). From gastrulation stage, it mainly resides in neural tissue. Ectopically expression of Sox31 mRNA leads to cyclopia, fusion eyes, or totally loss of anterior head structure, in accompany with severe notochord defects. Molecular markers indicate that forebrain tissue reduces sharply while the posterior neural tissue expands anteriorly. In addition, organizer specification is also suppressed. Oppositely, an antisense morpholino designed functionally knockdown Sox31 causes typically dorsalized phenotype and reversed central nervous system (CNS) anteroposterior (AP) patterning. Gain of function with chimeric construct, where Sox31 HMG DNA binding domain is fused to a transcription activation domain (VP16) or transcription suppression domain (EnR), suggests that Sox31 acts as a transcriptional suppressor in vivo. The expression of Bozozok (Dharma), a direct target gene of pre-MBT Wnt/beta-catenin signal, is suppressed by Sox31. Thus, to unveil the relationship between Sox31 and beta-catenin-related transcriptional activity, we designed Top/Fop luciferase assay in HEK293T cells, and found that Sox31 could indeed suppress Tcf/Lef-dependent transcriptional activity without influencing the stability of beta-catenin. Moreover, post-MBT Wnt signal was reduced in Sox31 morphants corresponding to the suppressed hindbrain structure, while phenotypic defects caused by excessive Sox31 could be rescued by Wnt antagonist dkk1. Taken together, Sox31 functions as an essential CNS AP patterning determinant and coordinates the CNS AP patterning process with organizer specification. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | HOMEOBOX GENE ; MIDBLASTULA TRANSITION ; MUTANT EMBRYOS ; DISTINCT ROLES ; EXPRESSION ; WNT ; SPECIFICATION ; NEUROECTODERM ; FOREBRAIN ; INDUCTION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000289836200008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/811]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Hu, SN,Wu, ZL,Yan, YC,et al. Sox31 is involved in central nervous system anteroposterior regionalization through regulating the organizer activity in zebrafish[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2011,43(5):387-399. |
| APA | Hu, SN,Wu, ZL,Yan, YC,&Li, YP.(2011).Sox31 is involved in central nervous system anteroposterior regionalization through regulating the organizer activity in zebrafish.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,43(5),387-399. |
| MLA | Hu, SN,et al."Sox31 is involved in central nervous system anteroposterior regionalization through regulating the organizer activity in zebrafish".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 43.5(2011):387-399. |
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