Identification of MicroRNAs and Application of RNA Interference for Gene Targeting In Vivo in the Rat Epididymis
文献类型：期刊论文
| 作者 | Zhang, YL; Zhang, JS; Zhou, YC; Zhao, Y; Ni, MJ |
| 刊名 | JOURNAL OF ANDROLOGY
 |
| 出版日期 | 2011 |
| 卷号 | 32期号:6页码:587-591 |
| 关键词 | RNAi microRNA sperm maturation DEFB SERPIN LCN |
| 通讯作者 | Zhang, YL (reprint author), 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,ylzhang@sibs.ac.cn |
| 英文摘要 | Identification, detection, and use of small-ribonucleic acid (RNA) molecules have been paid increasing attention in the past decades. Here we show studies of two interrelated lines of this research in a sperm maturation-related organ, the epididymis. First, by using microarray and small-RNA library screening systems, a series of spatially and temporally regulated known and novel small regulatory RNA molecules, referred to as microRNAs, has been identified and characterized. Second, small double-stranded RNAs, called small interfering RNAs, emerged as a powerful tool to knock down gene expression by a pathway known as RNA interference. It has been successfully used to silence epididymal gene expression in vivo to elucidate the function of several sperm motility- and capacitation-related proteins in the rat epididymis. |
| 学科主题 | Endocrinology & Metabolism |
| 类目[WOS] | Andrology |
| 关键词[WOS] | SPERM MATURATION ; BETA-DEFENSIN ; FAMILY ; THERAPEUTICS ; EXPRESSION ; PROTEINS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000296037400005 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/819]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, YL,Zhang, JS,Zhou, YC,et al. Identification of MicroRNAs and Application of RNA Interference for Gene Targeting In Vivo in the Rat Epididymis[J]. JOURNAL OF ANDROLOGY,2011,32(6):587-591. |
| APA | Zhang, YL,Zhang, JS,Zhou, YC,Zhao, Y,&Ni, MJ.(2011).Identification of MicroRNAs and Application of RNA Interference for Gene Targeting In Vivo in the Rat Epididymis.JOURNAL OF ANDROLOGY,32(6),587-591. |
| MLA | Zhang, YL,et al."Identification of MicroRNAs and Application of RNA Interference for Gene Targeting In Vivo in the Rat Epididymis".JOURNAL OF ANDROLOGY 32.6(2011):587-591. |
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