Recurrent trisomy and Robertsonian translocation of chromosome 14 in murine iPS cell lines
文献类型：期刊论文
| 作者 | Chen, Q; Shi, XY; Rudolph, C; Yu, Y; Zhang, D; Zhao, XY; Mai, S; Wang, G; Schlegelberger, B; Shi, QH |
| 刊名 | CHROMOSOME RESEARCH
 |
| 出版日期 | 2011 |
| 卷号 | 19期号:7页码:857-868 |
| 关键词 | Induced pluripotent stem cells Chromosome aberrations Robertsonian translocation Trisomy c-Myc |
| 通讯作者 | Shi, QH (reprint author), Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230027, Anhui, Peoples R China.,gwang22@sibs.ac.cn ; Schlegelberger.Brigitte@MH-Hannover.de ; qshi@ustc.edu.cn |
| 英文摘要 | Induced pluripotent stem (iPS) cells have greatly provoked people's interest due to their enormous potential of clinical applications. Increasing care is taken with the genetic safety of iPS cells. However, up to now, the chromosomal integrity of murine iPS (miPS) cells has been largely unknown. We have observed recurrent trisomy and/or Robertsonian translocation (Rb) of chromosome 14 in six out of nine independent miPS cell lines from three laboratories by G-banding, fluorescence in situ hybridization (FISH) and spectral karyotyping (SKY) analyses, while all the miPS cell lines were derived from mouse embryonic fibroblasts (MEFs) or neural precursor cells (NPCs) with a normal karyotype. The miPS cells with trisomy and/or Rb of chromosome 14 showed growth advantage over the miPS cells with a normal karyotype. We found a significantly higher frequency of Rbs in the miPS cell lines induced with c-Myc than those without c-Myc. Our findings demonstrate that miPS cell lines have the propensity for chromosomal aberrations and there is an obvious correlation between the extent of chromosomal aberrations in miPS cells and the transcriptional factors used for their reprogramming. Therefore, our study raises awareness of the need for improvements of the induction conditions of miPS cells in order to avoid the chromosomal aberrations and ensure future safe applications. |
| 学科主题 | Biochemistry & Molecular Biology; Genetics & Heredity |
| 类目[WOS] | Biochemistry & Molecular Biology ; Genetics & Heredity |
| 关键词[WOS] | PLURIPOTENT STEM-CELLS ; IN-SITU HYBRIDIZATION ; DOUBLE-STRAND BREAKS ; HUMAN SOMATIC-CELLS ; C-MYC ; GENE AMPLIFICATION ; COPY NUMBER ; DNA-DAMAGE ; MOUSE ; GENERATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000300089700002 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/829]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Chen, Q,Shi, XY,Rudolph, C,et al. Recurrent trisomy and Robertsonian translocation of chromosome 14 in murine iPS cell lines[J]. CHROMOSOME RESEARCH,2011,19(7):857-868. |
| APA | Chen, Q.,Shi, XY.,Rudolph, C.,Yu, Y.,Zhang, D.,...&Shi, QH.(2011).Recurrent trisomy and Robertsonian translocation of chromosome 14 in murine iPS cell lines.CHROMOSOME RESEARCH,19(7),857-868. |
| MLA | Chen, Q,et al."Recurrent trisomy and Robertsonian translocation of chromosome 14 in murine iPS cell lines".CHROMOSOME RESEARCH 19.7(2011):857-868. |
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