Hybridization kinetics analysis of an oligonucleotide microarray for microRNA detection
文献类型：期刊论文
| 作者 | Zhao, BT; Ding, S; Li, W; Jin, YX |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2011 |
| 卷号 | 43期号:7页码:551-555 |
| 关键词 | microRNA microarray microRNA |
| 通讯作者 | Zhao, BT (reprint author), Shanghai Univ, Sch Life Sci, Shanghai 200444, Peoples R China.,botaozhao@gmail.com ; yxjin@sibs.ac.cn |
| 英文摘要 | MicroRNA (miRNA) microarrays have been successfully used for profiling miRNA expression in many physiological processes such as development, differentiation, oncogenesis, and other disease processes. Detecting miRNA by miRNA microarray is actually based on nucleic acid hybridization between target molecules and their corresponding complementary probes. Due to the small size and high degree of similarity among miRNA sequences, the hybridization condition must be carefully optimized to get specific and reliable signals. Previously, we reported a microarray platform to detect miRNA expression. In this study, we evaluated the sensitivity and specificity of our microarray platform. After systematic analysis, we determined an optimized hybridization condition with high sensitivity and specificity for miRNA detection. Our results would be helpful for other hybridization-based miRNA detection methods, such as northern blot and nuclease protection assay. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | EXPRESSION ; RNA ; DIFFERENTIATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000292337100007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/831]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhao, BT,Ding, S,Li, W,et al. Hybridization kinetics analysis of an oligonucleotide microarray for microRNA detection[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2011,43(7):551-555. |
| APA | Zhao, BT,Ding, S,Li, W,&Jin, YX.(2011).Hybridization kinetics analysis of an oligonucleotide microarray for microRNA detection.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,43(7),551-555. |
| MLA | Zhao, BT,et al."Hybridization kinetics analysis of an oligonucleotide microarray for microRNA detection".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 43.7(2011):551-555. |
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