Quantitative analysis of expression of six BmGST genes in silkworm, Bombyx mori
文献类型：期刊论文
| 作者 | Zhao, GD; Zhang, YL; Gao, RN; Wang, RX; Zhang, T; Li, B; Zhang, Y; Lu, CD; Shen, WD; Wei, ZG |
| 刊名 | MOLECULAR BIOLOGY REPORTS
 |
| 出版日期 | 2011 |
| 卷号 | 38期号:8页码:4855-4861 |
| 关键词 | Bombyx mori BmGST genes Quantified transcription level Tissue-specific expression Induced expression |
| 通讯作者 | Shen, WD (reprint author), Soochow Univ, Preclin Med & Biol Sci Coll, 199 Renai Rd,Suzhou Ind Pk, Suzhou 215123, Jiangsu, Peoples R China.,szwei2002@126.com |
| 英文摘要 | Glutathione S-transferases (GSTs) are a multifunctional super gene family, some of which play an important role in insecticide resistance. In this research, we used a real-time quantitative RT-PCR method, and a novel strategy, to measure the transcriptional level per gene copy using an exogenous RNA reference and DNA reference. The transcription levels of six BmGST genes in different tissues of fifth instar Bombyx mori larvae and their responses to insecticide and fluoride were investigated. The results show different levels and patterns of expression of the different BmGSTs in the various tissues observed. The BmGSTs can be induced by insecticide and fluoride, but their responses to each are different. The results of this research are helpful in studying the tissue-specific expression of BmGSTs in Bombyx mori, and in developing new pesticide resistant silkworm varieties. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | GLUTATHIONE-S-TRANSFERASE ; MESSENGER-RNA ; IDENTIFICATION ; RESISTANCE ; FLUORIDE ; DEFENSE ; MIDGUT ; PCR |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000296013600004 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/835]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhao, GD,Zhang, YL,Gao, RN,et al. Quantitative analysis of expression of six BmGST genes in silkworm, Bombyx mori[J]. MOLECULAR BIOLOGY REPORTS,2011,38(8):4855-4861. |
| APA | Zhao, GD.,Zhang, YL.,Gao, RN.,Wang, RX.,Zhang, T.,...&Wei, ZG.(2011).Quantitative analysis of expression of six BmGST genes in silkworm, Bombyx mori.MOLECULAR BIOLOGY REPORTS,38(8),4855-4861. |
| MLA | Zhao, GD,et al."Quantitative analysis of expression of six BmGST genes in silkworm, Bombyx mori".MOLECULAR BIOLOGY REPORTS 38.8(2011):4855-4861. |
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