Comparative proteogenomic analysis of the Leptospira interrogans virulence-attenuated strain IPAV against the pathogenic strain 56601
文献类型：期刊论文
| 作者 | Zhong, Y; Chang, X; Cao, XJ; Zhang, Y; Zheng, HJ; Zhu, YZ; Cai, CS; Cui, ZL; Zhang, YY; Li, YY |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2011 |
| 卷号 | 21期号:8页码:1210-1229 |
| 关键词 | genome proteome Leptospira virulence mutation |
| 通讯作者 | Wang, SY (reprint author), Chinese Natl Human Genome Ctr Shanghai, Shanghai MOST Key Lab Hlth & Dis Genom, Shanghai 201203, Peoples R China.,wangsy@chgc.sh.cn ; yxli@sibs.ac.cn ; zr@sibs.ac.cn ; lixuan@sippe.ac.cn ; microbiology@sjtu.edu.cn |
| 英文摘要 | The virulence-attenuated Leptospira interrogans serovar Lai strain IPAV was derived by prolonged laboratory passage from a highly virulent ancestral strain isolated in China. We studied the genetic variations of IPAV that render it avirulent via comparative analysis against the pathogenic L. interrogans serovar Lai strain 56601. The complete genome sequence of the IPAV strain was determined and used to compare with, and then rectify and reannotate the genome sequence of strain 56601. Aside from their highly similar genomic structure and gene order, a total of 33 insertions, 53 deletions and 301 single-nucleotide variations (SNVs) were detected throughout the genome of IPAV directly affecting 101 genes, either in their 5' upstream region or within their coding region. Among them, the majority of the 44 functional genes are involved in signal transduction, stress response, transmembrane transport and nitrogen metabolism. Comparative proteomic analysis based on quantitative liquid chromatography (LC)-MS/MS data revealed that among 1 627 selected pairs of orthologs, 174 genes in the IPAV strain were upregulated, with enrichment mainly in classes of energy production and lipid metabolism. In contrast, 228 genes in strain 56601 were upregulated, with the majority enriched in the categories of protein translation and DNA replication/repair. The combination of genomic and proteomic approaches illustrated that altered expression or mutations in critical genes, such as those encoding a Ser/Thr kinase, carbon-starvation protein CstA, glutamine synthetase, GTP-binding protein BipA, ribonucleotide-diphosphate reductase and phosphate transporter, and alterations in the translational profile of lipoproteins or outer membrane proteins are likely to account for the virulence attenuation in strain IPAV. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | MASS-SPECTROMETRY ; MYCOBACTERIUM-TUBERCULOSIS ; GLUTAMINE-SYNTHETASE ; MODIFICATION SYSTEMS ; H+-PYROPHOSPHATASE ; GENOME ANNOTATION ; CRYSTAL-STRUCTURE ; ESCHERICHIA-COLI ; GENE-EXPRESSION ; ADP-RIBOSE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000293341100008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/840]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhong, Y,Chang, X,Cao, XJ,et al. Comparative proteogenomic analysis of the Leptospira interrogans virulence-attenuated strain IPAV against the pathogenic strain 56601[J]. CELL RESEARCH,2011,21(8):1210-1229. |
| APA | Zhong, Y.,Chang, X.,Cao, XJ.,Zhang, Y.,Zheng, HJ.,...&Guo, XK.(2011).Comparative proteogenomic analysis of the Leptospira interrogans virulence-attenuated strain IPAV against the pathogenic strain 56601.CELL RESEARCH,21(8),1210-1229. |
| MLA | Zhong, Y,et al."Comparative proteogenomic analysis of the Leptospira interrogans virulence-attenuated strain IPAV against the pathogenic strain 56601".CELL RESEARCH 21.8(2011):1210-1229. |
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