In vitro differentiation of rat embryonic stem cells into functional cardiomyocytes
文献类型：期刊论文
| 作者 | Cao, N; Liao, J; Liu, ZM; Zhu, WM; Wang, J; Liu, LJ; Yu, LL; Xu, P; Cui, C; Xiao, L |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2011 |
| 卷号 | 21期号:9页码:1316-1331 |
| 关键词 | rat ES cells differentiation cardiomyocytes |
| 通讯作者 | Yang, HT (reprint author), Chinese Acad Sci CAS, Inst Hlth Sci, Shanghai Inst Biol Sci SIBS, Key Lab Stem Cell Biol, Shanghai 200025, Peoples R China.,leixiao@sibs.ac.cn ; htyang@sibs.ac.cn |
| 英文摘要 | The recent breakthrough in the generation of rat embryonic stem cells (rESCs) opens the door to application of gene targeting to create models for the study of human diseases. In addition, the in vitro differentiation system from rESCs into derivatives of three germ layers will serve as a powerful tool and resource for the investigation of mammalian development, cell function, tissue repair, and drug discovery. However, these uses have been limited by the difficulty of in vitro differentiation. The aims of this study were to establish an in vitro differentiation system from rESCs and to investigate whether rESCs are capable of forming terminal-differentiated cardiomyocytes. Using newly established rESCs, we found that embryoid body (EB)-based method used in mouse ESC (mESC) differentiation failed to work for the serum-free cultivated rESCs. We then developed a protocol by combination of three chemical inhibitors and feeder-conditioned medium. Under this condition, rESCs formed EBs, propagated and differentiated into three embryonic germ layers. Moreover, rESC-formed EBs could differentiate into spontaneously beating cardiomyocytes after plating. Analyses of molecular, structural, and functional properties revealed that rESC-derived cardiomyocytes were similar to those derived from fetal rat hearts and mESCs. In conclusion, we successfully developed an in vitro differentiation system for rESCs through which functional myocytes were generated and displayed phenotypes of rat fetal cardiomyocytes. This unique cellular system will provide a new approach to study the early development and cardiac function, and serve as an important tool in pharmacological testing and cell therapy. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | CARDIOVASCULAR PROGENITOR CELLS ; CARDIAC PROGENITORS ; BLASTOCYSTS ; MODEL ; DERIVATION ; BODIES ; SYSTEM ; CULTURE ; LINES ; BODY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000294492500007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/850]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Cao, N,Liao, J,Liu, ZM,et al. In vitro differentiation of rat embryonic stem cells into functional cardiomyocytes[J]. CELL RESEARCH,2011,21(9):1316-1331. |
| APA | Cao, N.,Liao, J.,Liu, ZM.,Zhu, WM.,Wang, J.,...&Yang, HT.(2011).In vitro differentiation of rat embryonic stem cells into functional cardiomyocytes.CELL RESEARCH,21(9),1316-1331. |
| MLA | Cao, N,et al."In vitro differentiation of rat embryonic stem cells into functional cardiomyocytes".CELL RESEARCH 21.9(2011):1316-1331. |
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