Insulin-like growth factor binding protein-6 delays replicative senescence of human fibroblasts
文献类型：期刊论文
| 作者 | Micutkova, L; Diener, T; Li, C; Rogowska-Wrzesinska, A; Mueck, C; Huetter, E; Weinberger, B; Grubeck-Loebenstein, B; Roepstorff, P; Zeng, R |
| 刊名 | MECHANISMS OF AGEING AND DEVELOPMENT
 |
| 出版日期 | 2011 |
| 卷号 | 132期号:10页码:468-479 |
| 关键词 | IGFBP-6 Senescence Secretome Proteomics Fibroblast |
| 通讯作者 | Jansen-Duerr, P (reprint author), Austrian Acad Sci, Inst Biomed Aging Res, Rennweg 10, A-6020 Innsbruck, Austria.,p.jansen-duerr@oeaw.ac.at |
| 英文摘要 | Cellular senescence can be induced by a variety of mechanisms, and recent data suggest a key role for cytokine networks to maintain the senescent state. Here, we have used a proteomic LC-MS/MS approach to identify new extracellular regulators of senescence in human fibroblasts. We identified 26 extracellular proteins with significantly different abundance in conditioned media from young and senescent fibroblasts. Among these was insulin-like growth factor binding protein-6 (IGFBP-6), which was chosen for further analysis. When IGFBP-6 gene expression was downregulated, cell proliferation was inhibited and apoptotic cell death was increased. Furthermore, downregulation of IGFBP-6 led to premature entry into cellular senescence. Since IGFBP-6 overexpression increased cellular lifespan, the data suggest that IGFBP-6, in contrast to other IGF binding proteins, is a negative regulator of cellular senescence in human fibroblasts. (C) 2011 Elsevier Ireland Ltd. All rights reserved. |
| 学科主题 | Cell Biology; Geriatrics & Gerontology |
| 类目[WOS] | Cell Biology ; Geriatrics & Gerontology |
| 关键词[WOS] | QUIESCENT HUMAN FIBROBLASTS ; HUMAN CEREBROSPINAL-FLUID ; HUMAN-DIPLOID FIBROBLASTS ; ENDOTHELIAL-CELLS ; CELLULAR SENESCENCE ; IN-VIVO ; PREMATURE SENESCENCE ; MASS-SPECTROMETRY ; GENE-EXPRESSION ; CARCINOMA CELLS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000296216100002 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/856]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Micutkova, L,Diener, T,Li, C,et al. Insulin-like growth factor binding protein-6 delays replicative senescence of human fibroblasts[J]. MECHANISMS OF AGEING AND DEVELOPMENT,2011,132(10):468-479. |
| APA | Micutkova, L.,Diener, T.,Li, C.,Rogowska-Wrzesinska, A.,Mueck, C.,...&Jansen-Duerr, P.(2011).Insulin-like growth factor binding protein-6 delays replicative senescence of human fibroblasts.MECHANISMS OF AGEING AND DEVELOPMENT,132(10),468-479. |
| MLA | Micutkova, L,et al."Insulin-like growth factor binding protein-6 delays replicative senescence of human fibroblasts".MECHANISMS OF AGEING AND DEVELOPMENT 132.10(2011):468-479. |
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