Site-directed mutagenesis of aromatic residues in the carbohydrate-binding module of Bacillus endoglucanase EGA decreases enzyme thermostability
文献类型：期刊论文
| 作者 | Yin, QY; Teng, YG; Ding, M; Zhao, FK |
| 刊名 | BIOTECHNOLOGY LETTERS
 |
| 出版日期 | 2011 |
| 卷号 | 33期号:11页码:2209-2216 |
| 关键词 | Aromatic amino acid Carbohydrate-binding module Cellulase Hydrophobic interaction Site-directed mutagenesis Thermostability |
| 通讯作者 | Zhao, FK (reprint author), Chinese Acad Sci, Grad Sch, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,fkzhao@sibs.ac.cn |
| 英文摘要 | The endoglucanase, EGA, fromBacillus sp. AC-1 comprises a glycosyl hydrolase family-9 catalytic module (CM9) and a family-3 carbohydrate-binding module (CBM3). Seven aromatic residues were subjected to site-directed mutagenesis in both CBM3 and EGA to investigate their roles in enzyme thermostability. The complexes generated by mixing CBMY527G, CBMW532A, or CBMF592G with CM9 each lost their activities after 15 min at 45 degrees C, while the wild-type complex retained >70% activity after 2 h. The mutants EGAY527G, EGAW532A, and EGAF592G showed little activity after 15 min at 60 degrees C, whereas EGA remained 70% active after 2 h. Thus the residues Tyr(527), Trp(532), and Phe(592) contribute not only to CBM3-mediated stability of CM9 but also to EGA thermostability suggesting that hydrophobic interaction between the two modules, independent of covalent linkages, is important for enzyme thermostability. |
| 学科主题 | Biotechnology & Applied Microbiology |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | SWISS-MODEL ; CELLULOSE ; XYLANASE ; DOMAINS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000297163000014 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/866]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yin, QY,Teng, YG,Ding, M,et al. Site-directed mutagenesis of aromatic residues in the carbohydrate-binding module of Bacillus endoglucanase EGA decreases enzyme thermostability[J]. BIOTECHNOLOGY LETTERS,2011,33(11):2209-2216. |
| APA | Yin, QY,Teng, YG,Ding, M,&Zhao, FK.(2011).Site-directed mutagenesis of aromatic residues in the carbohydrate-binding module of Bacillus endoglucanase EGA decreases enzyme thermostability.BIOTECHNOLOGY LETTERS,33(11),2209-2216. |
| MLA | Yin, QY,et al."Site-directed mutagenesis of aromatic residues in the carbohydrate-binding module of Bacillus endoglucanase EGA decreases enzyme thermostability".BIOTECHNOLOGY LETTERS 33.11(2011):2209-2216. |
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