Over-expression of Slit2 induces vessel formation and changes blood vessel permeability in mouse brain
文献类型：期刊论文
| 作者 | Han, HX; Geng, JG |
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2011 |
| 卷号 | 32期号:11页码:1327-1336 |
| 关键词 | Slit2 blood-brain barrier permeability vessel endothelial cell tight junction |
| 通讯作者 | Geng, JG (reprint author), Chinese Acad Sci, Mol Cell Biol Lab, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,genglaboratory@gmail.com |
| 英文摘要 | Aim: To investigate the effect of the axon guidance cue Slit2 on the density of blood vessels and permeability of the blood-brain barrier in mouse brain. Methods: hSlit2 transgenic mouse line was constructed, and the phenotypes of the mice were compared with wild-type mice in respect to the lateral ventricle (LV), ventricle pressure, and the choroids plexus. An in vivo Miles permeability assay and an amyloid-beta permeability assay were used to assess the permeability of brain blood vessels. Brain vessel casting and intracerebral hemorrhage models were built to investigate vessel density in the transgenic mice. An in vitro permeability assay was used to test whether Slit2 could change the permeability and tight junctions of blood vessel endothelial cells. Results: Hydrocephalus occurred in some transgenic mice, and a significantly larger lateral ventricle area and significantly higher ventricle pressure were observed in the transgenic mice. The transgenic mice displayed changed construction of the choroids plexus, which had more micro vessels, dilated vessels, gaps between epithelial cells and endothelial cells than wild-type mice. Slit2 significantly increased brain vessel density and the permeability of brain vessels to large molecules. These blood vessels were more sensitive to cues that induce brain hemorrhage. At the cellular level, Slit2 disturbed the integrity of tight junctions in blood vessel endothelial cells and improved the permeability of the endothelial cell layer. Thus, it promoted the entry of amyloid-beta peptides from the serum into the central nervous system, where they bound to neurons. Conclusion: Slit2 increases vessel density and permeability in the brains of transgenic mice. Thus, Slit2 induces numerous changes in brain vessels and the barrier system. |
| 学科主题 | Chemistry; Pharmacology & Pharmacy |
| 类目[WOS] | Chemistry, Multidisciplinary ; Pharmacology & Pharmacy |
| 关键词[WOS] | ENDOTHELIAL GROWTH-FACTOR ; REPULSIVE AXON GUIDANCE ; CELL-MIGRATION ; TUMOR ANGIOGENESIS ; ROUNDABOUT ; REPELLENT ; RECEPTORS ; VEGF ; INFLAMMATION ; CHEMOTAXIS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000296739400004 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/868]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Han, HX,Geng, JG. Over-expression of Slit2 induces vessel formation and changes blood vessel permeability in mouse brain[J]. ACTA PHARMACOLOGICA SINICA,2011,32(11):1327-1336. |
| APA | Han, HX,&Geng, JG.(2011).Over-expression of Slit2 induces vessel formation and changes blood vessel permeability in mouse brain.ACTA PHARMACOLOGICA SINICA,32(11),1327-1336. |
| MLA | Han, HX,et al."Over-expression of Slit2 induces vessel formation and changes blood vessel permeability in mouse brain".ACTA PHARMACOLOGICA SINICA 32.11(2011):1327-1336. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。