Intracerebroventricular transplantation of human amniotic epithelial cells ameliorates spatial memory deficit in the doubly transgenic mice coexpressing APPswe and PS1 Delta E9-deleted genes
文献类型：期刊论文
| 作者 | Xue, SR; Chen, CF; Dong, WL; Hui, GZ; Liu, TJ; Guo, LH |
| 刊名 | CHINESE MEDICAL JOURNAL
 |
| 出版日期 | 2011 |
| 卷号 | 124期号:17页码:2642-2648 |
| 关键词 | Alzheimer's disease human amniotic epithelial cells transgenic mice spatial memory deficit |
| 通讯作者 | Guo, LH (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,huiguozhen@163.com ; guolihe@cellstar.com.cn |
| 英文摘要 | Background Human amniotic epithelial cells (HAECs), which have characteristics of both embryonic and pluripotent stem cells, are therefore a candidate in cell therapy without creating legal or ethical problems. In the present study, we aimed to investigate the effects of intracerebroventricular transplantation of HAECs on doubly transgenic mice of Alzheimer's disease (AD) coexpressing presenilin-1 (PS 1) and mutant Sweden amyloid precursor protein (APPswe) genes. Methods The offspring mice genotypes were detected using PCR identification of APPswe and PS1 gene. The doubly transgenic (TG) mice (n=20) and wild-type (WT) mice (n=20) were randomly divided into two groups respectively: the transplantation group treated with HAECs and the control group with phosphate buffered saline. Six radial arm water maze test was used to assess the spatial memory in the TG and WT mice. Amyloid plaques and neurofibrillary tangles were analyzed using congo red and acid-silver methenamine staining respectively. Immunofluorescence cytochennistry was used to track the survival of HAECs. Immunohistochemistry was used to determine the expression of octamer-binding protein 4 (Oct-4) and Nanog in the HAECs. High performance liquid chromatography was used to measure acetylcholine in hippocampus. The density of cholinergic neurons in basal forebrain and nerve fibers in hippocampus was measured using acetylcholinesterase staining. Results Amyloid deposition occurred in hippocampus and frontal cortex in the double TG mice aged 8 months, but not in WT mice. The results also showed that transplanted HAECs can survive for at least 8 weeks and migrate to the third ventricle without immune rejection. The graft HAECs can also express the specific marker Oct-4 and Nanog of stem cell. Compared with the control group, transplantation of HAECs can not only significantly improve the spatial memory of the TG mice, but also increase acetylcholine concentration and the number of hippocampal cholinergic neurites. Conclusions These results demonstrate that intracerebroventricular transplantation of HAECs can improve the spatial memory of the double TG mice. The higher content of acetylcholine in hippocampus released by more survived cholinergic neurites is one of the causes of this improvement. Chin Med J 2011;124(17):2642-2648 |
| 学科主题 | General & Internal Medicine |
| 类目[WOS] | Medicine, General & Internal |
| 关键词[WOS] | ALZHEIMERS-DISEASE ; MOUSE MODEL ; STEM-CELLS ; DIFFERENTIATION ; DYSFUNCTION ; EXPRESSION ; HYPOTHESIS ; THERAPY ; PROMOTE ; NEURONS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000295297400014 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/878]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Xue, SR,Chen, CF,Dong, WL,et al. Intracerebroventricular transplantation of human amniotic epithelial cells ameliorates spatial memory deficit in the doubly transgenic mice coexpressing APPswe and PS1 Delta E9-deleted genes[J]. CHINESE MEDICAL JOURNAL,2011,124(17):2642-2648. |
| APA | Xue, SR,Chen, CF,Dong, WL,Hui, GZ,Liu, TJ,&Guo, LH.(2011).Intracerebroventricular transplantation of human amniotic epithelial cells ameliorates spatial memory deficit in the doubly transgenic mice coexpressing APPswe and PS1 Delta E9-deleted genes.CHINESE MEDICAL JOURNAL,124(17),2642-2648. |
| MLA | Xue, SR,et al."Intracerebroventricular transplantation of human amniotic epithelial cells ameliorates spatial memory deficit in the doubly transgenic mice coexpressing APPswe and PS1 Delta E9-deleted genes".CHINESE MEDICAL JOURNAL 124.17(2011):2642-2648. |
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