Expression analysis and characteristics of hypothetical protein BmLOC778500 from silkworm, Bombyx mori
文献类型：期刊论文
| 作者 | Zhang, WP; Lan, HL; Wu, T; Jiang, CY; Lv, ZB; Nie, ZM; Wu, XF; Zhang, YZ |
| 刊名 | AFRICAN JOURNAL OF BIOTECHNOLOGY
 |
| 出版日期 | 2011 |
| 卷号 | 10期号:19页码:3753-3761 |
| 关键词 | BmLOC778500 Bombyx mori expression analysis tissue distribution sub-cellular localization |
| 通讯作者 | Zhang, YZ (reprint author), Zhejiang Sci & Technol Univ, Inst Biochem, Coll Life Sci, Hangzhou 310018, Zhejiang, Peoples R China.,yaozhou@zstu.edu.cn |
| 英文摘要 | We cloned a cDNA from silkworm pupal cDNA library and found that, it encodes LOC778500 protein (a "hypothetical protein" in the National Center for Biotechnology Information (NCBI) database). Thus, we named it BmLOC778500 (Bombyx mori LOC778500). It contains an open reading frame of 489 bp encoding a polypeptide of 162 amino acid residues with a predicted molecular mass of 19.4 kDa. The gene codes for a protein homologous to hypothetical proteins of Tribolium castaneum, Pediculus humcorporisanus and Drosophila erecta among others. Purified BmLOC778500 was used to generate anti-BmLOC778500 polyclonal antibody for determining tissue distribution and sub-cellular localization of BmLOC778500. Western blotting analysis revealed that, BmLOC778500 proteins existed in Malpighian tubule of fifth-instar larva of B. mori. Immunostaining indicated that, BmLOC778500 proteins are mainly located in cytosol and nucleus as well as in not-dividing cells, whereas are distributed in cell membrane in dividing cells. These results have provided important information for further function studies of BmLOC778500 protein. |
| 学科主题 | Biotechnology & Applied Microbiology |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | CELL-MEDIATED-IMMUNITY ; COMPARATIVE SELECTIVITY ; CORYNEBACTERIUM-PARVUM ; BORDETELLA-PERTUSSIS ; COMPLETE ADJUVANTS ; MURAMYL DIPEPTIDE ; RHGM-CSF ; SEQUENCE ; GENOME ; ALHYDROGEL |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000290668200014 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/880]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, WP,Lan, HL,Wu, T,et al. Expression analysis and characteristics of hypothetical protein BmLOC778500 from silkworm, Bombyx mori[J]. AFRICAN JOURNAL OF BIOTECHNOLOGY,2011,10(19):3753-3761. |
| APA | Zhang, WP.,Lan, HL.,Wu, T.,Jiang, CY.,Lv, ZB.,...&Zhang, YZ.(2011).Expression analysis and characteristics of hypothetical protein BmLOC778500 from silkworm, Bombyx mori.AFRICAN JOURNAL OF BIOTECHNOLOGY,10(19),3753-3761. |
| MLA | Zhang, WP,et al."Expression analysis and characteristics of hypothetical protein BmLOC778500 from silkworm, Bombyx mori".AFRICAN JOURNAL OF BIOTECHNOLOGY 10.19(2011):3753-3761. |
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