Smad6 promotes neuronal differentiation in the intermediate zone of the dorsal neural tube by inhibition of the Wnt/beta-catenin pathway
文献类型：期刊论文
| 作者 | Xie, ZH; Chen, YF; Li, ZF; Bai, G; Zhu, Y; Yan, R; Tan, FZ; Chen, YG; Guillemot, F; Li, L |
| 刊名 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
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| 出版日期 | 2011 |
| 卷号 | 108期号:29页码:12119-12124 |
| 关键词 | intermediate zone neurogenesis |
| 通讯作者 | Jing, NH (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Cell Biol, Shanghai 200031, Peoples R China.,njing@sibs.ac.cn |
| 英文摘要 | Proliferation of the neural/neuronal progenitor cells (NPCs) at the ventricular zone of the dorsal spinal cord requires the stimuli of Wnt and bone morphogenic protein (BMP). However, how these two signaling pathways are regulated to initiate differentiation in the NPCs as they enter the intermediate zone is not known. Here, we show that Smad6, a negative regulator of BMP signaling, is expressed in the intermediate zone of the chick dorsal spinal cord. Knockdown experiments show that Smad6 is required for promoting NPCs to exit the cell cycle and differentiate into neurons. Although we find that Smad6 inhibits BMP signaling, as expected, we also find that Smad6 unexpectedly inhibits the Wnt/beta-catenin pathway. The inhibition of the Wnt/beta-catenin pathway by Smad6 is independent of its effect on the BMP pathway. Rather, Smad6 through its N-terminal domain and link region enhances the interaction of C-terminal binding protein with the beta-catenin/T cell factor (TCF) complex and the TCF-binding element to inhibit beta-cateninmediated transcriptional activation. Our study provides evidence that transition of NPCs from a proliferative state to a differentiating state is controlled by the dual inhibitory role of Smad6 to both BMP and Wnt signaling at the level of transcription. |
| 学科主题 | Science & Technology - Other Topics |
| 类目[WOS] | Multidisciplinary Sciences |
| 关键词[WOS] | CENTRAL-NERVOUS-SYSTEM ; CELL-CYCLE EXIT ; TRANSCRIPTION FACTOR ; BETA-CATENIN ; TUMOR-SUPPRESSOR ; PROGENITOR CELLS ; PRONEURAL GENES ; CEREBRAL-CORTEX ; SPINAL-CORD ; STEM-CELLS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000292876900079 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/887]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Xie, ZH,Chen, YF,Li, ZF,et al. Smad6 promotes neuronal differentiation in the intermediate zone of the dorsal neural tube by inhibition of the Wnt/beta-catenin pathway[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2011,108(29):12119-12124. |
| APA | Xie, ZH.,Chen, YF.,Li, ZF.,Bai, G.,Zhu, Y.,...&Jing, NH.(2011).Smad6 promotes neuronal differentiation in the intermediate zone of the dorsal neural tube by inhibition of the Wnt/beta-catenin pathway.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,108(29),12119-12124. |
| MLA | Xie, ZH,et al."Smad6 promotes neuronal differentiation in the intermediate zone of the dorsal neural tube by inhibition of the Wnt/beta-catenin pathway".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 108.29(2011):12119-12124. |
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