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Tet-Mediated Formation of 5-Carboxylcytosine and Its Excision by TDG in Mammalian DNA
文献类型：期刊论文
| 作者 | He, YF; Li, BZ; Li, Z; Liu, P; Wang, Y; Tang, QY; Ding, JP; Jia, YY; Chen, ZC; Li, L |
| 刊名 | SCIENCE
 |
| 出版日期 | 2011 |
| 卷号 | 333期号:6047页码:1303-1307 |
| 通讯作者 | Xu, GL (reprint author), Chinese Acad Sci, Grp DNA Metab, State Key Lab Mol Biol, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,glxu@sibs.ac.cn |
| 英文摘要 | The prevalent DNA modification in higher organisms is the methylation of cytosine to 5-methylcytosine (5mC), which is partially converted to 5-hydroxymethylcytosine (5hmC) by the Tet (ten eleven translocation) family of dioxygenases. Despite their importance in epigenetic regulation, it is unclear how these cytosine modifications are reversed. Here, we demonstrate that 5mC and 5hmC in DNA are oxidized to 5-carboxylcytosine (5caC) by Tet dioxygenases in vitro and in cultured cells. 5caC is specifically recognized and excised by thymine-DNA glycosylase (TDG). Depletion of TDG in mouse embyronic stem cells leads to accumulation of 5caC to a readily detectable level. These data suggest that oxidation of 5mC by Tet proteins followed by TDG-mediated base excision of 5caC constitutes a pathway for active DNA demethylation. |
| 学科主题 | Science & Technology - Other Topics |
| 类目[WOS] | Multidisciplinary Sciences |
| 关键词[WOS] | EMBRYONIC STEM-CELLS ; GLYCOSYLASE ACTIVITY ; GENE-EXPRESSION ; 5-HYDROXYMETHYLCYTOSINE ; DEMETHYLATION ; METHYLATION ; REPAIR ; DEAMINATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000294406400058 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/894]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | He, YF,Li, BZ,Li, Z,et al. Tet-Mediated Formation of 5-Carboxylcytosine and Its Excision by TDG in Mammalian DNA[J]. SCIENCE,2011,333(6047):1303-1307. |
| APA | He, YF.,Li, BZ.,Li, Z.,Liu, P.,Wang, Y.,...&Xu, GL.(2011).Tet-Mediated Formation of 5-Carboxylcytosine and Its Excision by TDG in Mammalian DNA.SCIENCE,333(6047),1303-1307. |
| MLA | He, YF,et al."Tet-Mediated Formation of 5-Carboxylcytosine and Its Excision by TDG in Mammalian DNA".SCIENCE 333.6047(2011):1303-1307. |
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