Synthetic osteogenic growth peptide promotes differentiation of human bone marrow mesenchymal stem cells to osteoblasts via RhoA/ROCK pathway
文献类型：期刊论文
| 作者 | Chen, ZX; Wang, XF; Shao, YC; Shi, DY; Chen, TY; Cui, DF; Jiang, XX |
| 刊名 | MOLECULAR AND CELLULAR BIOCHEMISTRY
 |
| 出版日期 | 2011 |
| 卷号 | 358期号:1页码:221-227 |
| 关键词 | Osteogenic growth peptide Osteoblastogenesis Bone marrow stromal cells Cell differentiation |
| 通讯作者 | Jiang, XX (reprint author), Fudan Univ, Dept Orthoped Surg, Shanghai Zhongshan Hosp, 180 Fenglin Rd, Shanghai 200032, Peoples R China.,Jiang_xiaoxing@126.com |
| 英文摘要 | The osteogenic growth peptide (OGP) is a naturally occurring tetradecapeptide that has attracted considerable clinical interest as a bone anabolic agent and hematopoietic stimulator. In vitro studies have demonstrated that OGP directly regulates the bone marrow mesenchymal stem cells' (BMSCs) differentiation into osteoblasts. However, the exact mechanism of this process remains unknown. In the present study, we investigated the role of RhoA/ROCK signaling in differentiation along this lineage using human BMSCs. OGP treatment increased the mRNA level of bone morphogenetic protein-2 and alkaline phosphatase activity after osteogenic induction. Analysis of BMSCs induced in the presence of OGP revealed an increase in RhoA activity, and phosphorylation of FAK and cofilin. The ROCK-specific inhibitors, Y27632, blocked the OGP-induced regulation of BMSC differentiation. Taken together, these data suggest that OGP not only acts on BMSCs to stimulate osteogenic differentiation, but also in a dose-dependent manner, and this effect is mediated via the activation of RhoA/ROCK pathway. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | C-TERMINAL PENTAPEPTIDE ; RHO-KINASE ; OGP(10-14) ACTS ; PROTEIN-KINASE ; IN-VITRO ; EXPRESSION ; OSTEOPOROSIS ; INVOLVEMENT ; INDUCTION ; LINEAGE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000296286700025 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/898]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Chen, ZX,Wang, XF,Shao, YC,et al. Synthetic osteogenic growth peptide promotes differentiation of human bone marrow mesenchymal stem cells to osteoblasts via RhoA/ROCK pathway[J]. MOLECULAR AND CELLULAR BIOCHEMISTRY,2011,358(1):221-227. |
| APA | Chen, ZX.,Wang, XF.,Shao, YC.,Shi, DY.,Chen, TY.,...&Jiang, XX.(2011).Synthetic osteogenic growth peptide promotes differentiation of human bone marrow mesenchymal stem cells to osteoblasts via RhoA/ROCK pathway.MOLECULAR AND CELLULAR BIOCHEMISTRY,358(1),221-227. |
| MLA | Chen, ZX,et al."Synthetic osteogenic growth peptide promotes differentiation of human bone marrow mesenchymal stem cells to osteoblasts via RhoA/ROCK pathway".MOLECULAR AND CELLULAR BIOCHEMISTRY 358.1(2011):221-227. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。