The Tight Junction Protein, Occludin, Regulates the Directional Migration of Epithelial Cells
文献类型：期刊论文
| 作者 | Du, D; Xu, FL; Yu, LH; Zhang, CY; Lu, XF; Yuan, HX; Huang, Q; Zhang, F; Bao, HY; Jia, LH |
| 刊名 | DEVELOPMENTAL CELL
 |
| 出版日期 | 2010 |
| 卷号 | 18期号:1页码:52-63 |
| 通讯作者 | Chen, ZJ (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,zjchen@sibs.ac.cn |
| 英文摘要 | Cell polarity proteins regulate tight junction formation and directional migration in epithelial cells. To date, the mechanism by which these polarity proteins assemble at the leading edge of migrating epithelial cells remains unclear. We report that occludin, a transmembrane protein, is localized at the leading edge of migrating cells and regulates directional cell migration. During migration, occludin knockdown disrupted accumulation of aPKC-Par3 and PATJ at the leading edge, and led to a disorganized microtubule network and defective reorientation of the microtubule organization center (MTOC). Phosphorylation of occludin at tyrosine 473 residue allowed recruitment of p85 alpha to the leading edge via association with its C-terminal SH2 domain. Loss of occludin attenuated activation of PI3K, leading to disorganization of the actin cytoskeleton and reduced cell protrusions. Our data indicate that occludin is required for the leading-edge localization of polarity proteins aPKC-Par3 and PATJ and promotes cell protrusion by regulating membrane-localized activation of PI3K. |
| 学科主题 | Cell Biology ; Developmental Biology |
| 类目[WOS] | Cell Biology ; Developmental Biology |
| 关键词[WOS] | DISTINCT ROLES ; RHO-GTPASES ; TYROSINE PHOSPHORYLATION ; MEMBRANE-PROTEIN ; OXIDATIVE STRESS ; LEADING-EDGE ; POLARITY ; CDC42 ; POLARIZATION ; COMPLEX |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000273829800008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/922]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Du, D,Xu, FL,Yu, LH,et al. The Tight Junction Protein, Occludin, Regulates the Directional Migration of Epithelial Cells[J]. DEVELOPMENTAL CELL,2010,18(1):52-63. |
| APA | Du, D.,Xu, FL.,Yu, LH.,Zhang, CY.,Lu, XF.,...&Chen, ZJ.(2010).The Tight Junction Protein, Occludin, Regulates the Directional Migration of Epithelial Cells.DEVELOPMENTAL CELL,18(1),52-63. |
| MLA | Du, D,et al."The Tight Junction Protein, Occludin, Regulates the Directional Migration of Epithelial Cells".DEVELOPMENTAL CELL 18.1(2010):52-63. |
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