Soluble expression and characterization of a mouse epididymis-specific protein lipocalin6
文献类型：期刊论文
| 作者 | Guo, CY; Lian, YD; Liu, Q; Liu, JF; Zhang, YL; Lin, DH |
| 刊名 | PROTEIN EXPRESSION AND PURIFICATION
 |
| 出版日期 | 2010 |
| 卷号 | 69期号:1页码:64-67 |
| 关键词 | Lipocalin6 Soluble expression Stability GB1 Circular dichroism Fluorescence |
| 通讯作者 | Lin, DH (reprint author), Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Inst Biol Sci, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China.,dhlin@mail.shcnc.ac.cn |
| 英文摘要 | Mouse lipocalin6 (mLcn6) was recently identified to be specifically expressed in the epididymis and speculated to may play a role in sperm maturation. However, further studies were hindered due to the bottleneck to obtain enough recombinant mLcn6 proteins. In this article, GB1 tag was successfully applied to improve the soluble expression of mLcn6. Thermal unfolding experiments demonstrate that GB1 can enhance the structural stability of mLcn6. Fluorescence spectroscopy experiments show that mLcn6 prepared according to our procedure has high affinities to both retinoic acid (K(d) = 810 nM) and retinol (K(d) = 210 nM). In conclusion, soluble, stable and active mLcn6 was recombinantly prepared with the help of the GB1 tag, which will facilitate the structural and functional studies of mLcn6. (C) 2009 Elsevier Inc. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 关键词[WOS] | RETINOL-BINDING-PROTEIN ; PROSTAGLANDIN D SYNTHASE ; MARKER ; PURIFICATION ; REGION ; CELLS ; LIVER |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000272103200010 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/939]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Guo, CY,Lian, YD,Liu, Q,et al. Soluble expression and characterization of a mouse epididymis-specific protein lipocalin6[J]. PROTEIN EXPRESSION AND PURIFICATION,2010,69(1):64-67. |
| APA | Guo, CY,Lian, YD,Liu, Q,Liu, JF,Zhang, YL,&Lin, DH.(2010).Soluble expression and characterization of a mouse epididymis-specific protein lipocalin6.PROTEIN EXPRESSION AND PURIFICATION,69(1),64-67. |
| MLA | Guo, CY,et al."Soluble expression and characterization of a mouse epididymis-specific protein lipocalin6".PROTEIN EXPRESSION AND PURIFICATION 69.1(2010):64-67. |
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